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Monkeypox Virus Real Time PCR Kit
Intended Use
This kit is used for in vitro qualitative nucleic acid detection of
Monkeypox Virus in Human serum, lesion exudate samples and swab
specimens. The test results of this kit are for clinical reference
only and should not be used as the only standard for clinical
diagnosis.
Monkeypox is a rare zoonotic disease characterized by skin
eruptions caused by the Monkeypox virus (MPV) and whose clinical
symptoms are similar to smallpox. Transmitted mainly through
animals, humans can contract monkeypox from bites from infected
animals or from direct contact with the blood, fluids and rashes of
infected animals, and the virus can also be transmitted from person
to person.
Specification
Product Name | Monkeypox Virus Real Time PCR Kit |
Detection Channels | FAM |
IC | VIC |
Ct Value | 35 |
Limit of Detection | 200 Copies/mL |
Storage | -20℃±5℃ |
Transportation | Under frozen condition |
Shelf Life | 12 Months |
Sample Type | Nasal swabs, oropharyngeal swab, saliva, urine, lesion tissue, exudate, and blood, etc. |
Package | 24 Tests/Kit,48 Tests/Kit,96 Tests/Kit |
Components | PCR Reaction Buffer, PCR Enzyme Mixture, Positive control, Negative control |
TEST PRINCIPLE
This kit adopts real time fluorescence PCR technology, which is
suitable for nucleic acid detection of monkeypox virus extracted
from clinical samples such as Human serum, lesion exudate samples
and swab specimens Each reaction system contains specific primers
and fluorescent probes to detect the virus, and the monkeypox virus
nucleic acid can be qualitatively detected by collecting the
fluorescent signal generated by PCR amplification.
In addition, specific primers and fluorescent probes for the
internal standard control for human clinical specimen detection are
added to each reaction system, and the collection, transportation
and extraction process of the sample to be measured is monitored,
indicating the false negative of the test result.
Real-Time PCR System: Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5,Hongshi SLAN-96S/96P, AGS8830, AGS4800.
COMPONENTS
Specifications | LQ-000301 24T | LQ-000302 48T | LQ-000303 96T |
PCR Reaction Buffer | 336μL×1 tube | 672μL×1 tube | 672μL×2 tube |
PCR Enzyme Mixture | 30μL×1 tube | 50μL×1 tube | 100μL×1 tube |
Positive control | 50μL×1 tube | 100μL×1 tube | 200μL×1 tube |
Negative control | 50μL×1 tube | 100μL×1 tube | 200μL×1 tube |
Instruction for use (pcs) | 1 | 1 | 1 |