Rotavirus+Cryptosporidia+Coronavirus
+E. Coli Antigen Combo Rapid Test (Feces)
REF VIRCCE-647
INTENDED USE
The Rotavirus+Cryptosporidia+Coronavirus+E. Coli Antigen Combo
Rapid Test (Feces) is a sandwich lateral flow immunochromatographic
assay for the qualitative detection of Rotavirus, Cryptosporidia,
Coronavirus, and E. Coli antigens in Bovine’s feces.
For veterinary use only.
PRINCIPLE
The Rotavirus+Cryptosporidia+Coronavirus+E. Coli Antigen Combo
Antigen Rapid Test is based on sandwich lateral flow
immunochromatographic assay. The test has four testing windows with
an invisible T (test) zone and C (control) zone in each. During
testing, the liquid will laterally flow on the surface of the test
strip. If there is enough Rotavirus, Cryptosporidia, Coronavirus,
and E. Coli antigens in the sample, a visible T line will appear in
the corresponding region. The C line should always appear after a
sample is applied, indicating a valid result. By thismeans, the
test can accurately indicate the presence of Rotavirus,
Cryptosporidia,
Coronavirus, and E. Coli antigens in the samplE.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or
refrigerated (2-30 °C). The test is stable through the expiration
date printed on the sealed pouch. The test must remain in the
sealed pouch until use. DO NOT FREEZE.
Do not use beyond the expiration date.
PRECAUTIONS
Do not use after the expiration date. Do not reuse the test kit.
Handle all specimens as if they contain infectious agents.
Observe established precautions against microbiological hazards
throughout testing and follow the standard procedures for proper
disposal of specimens.
Wear disposable gloves and eye protection when specimens are
being tested.
Humidity and temperature can adversely affect results.
Do not remove test from its pouch until immediately before use.
Do not mix components from different lot and different products.
Please ensure that an appropriate amount of samples are used for
testing. Too much or too little sample size may lead to deviation
of results.
MATERIALS
Materials Provided
• Test Cups (with dilution buffer)
• Swabs
• Package Insert
Materials Required But Not Provided
• Timer
DIRECTIONS FOR USE
Allow the test and specimen to equilibrate to room temperature
(15-30 °C) prior to testing.
1. Remove the test from the foil pouch and use it as soon as
possible. Best results will be obtained if the assay is performed
within one hour.
2. Unscrew the cap of the test cup.
Collect bovine’s feces with the swab from bovine’s anus. Insert
the wet swab into the test cup and mix well. Remove the swab.
Collect bovine’s feces with the collection applicator on the
ground. Randomly stab the specimen collection applicator into the
specimen in at least
3 different sites to collect approximately 50 mg of feces
(equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
3. Insert the specimen collection applicator back into the test cup
and tighten the cap.
4. Shake the test cup for about 10-15 seconds to mix well. Leave
the cup for Raction for 2 minutes.
5. Remove the plastic limit strip of the test cup.
6. Put the test cup on a clean and level surface, press the cup
body from the top to the bottom and start the timer.
Note: Keep the test upright while testing. Do not move or turn the
test upside down.
7. Read the result at 10 minutes. Do not interpret results after 15
minutes.