10T Calprotectin And Lactoferrin Test For Inflammatory Gastrointestinal Disorders
Brand Name:Citest
Certification:CE
Model Number:OIBD-625
Minimum Order Quantity:N/A
Delivery Time:2-4 Weeks
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Product Details
Calprotectin and Lactoferrin Comb lateral flow Rapid Test Cassette
in human feces CE Certificated
| Principle | Chromatographic Immunoassay |
| Format | Cassette |
| Specimen | Feces |
| Certificate | CE |
| Reading Time | 5 minutes |
| Pack | 10 T |
| Storage Temperature | 2-30°C |
| Shelf Life | 2 Years |
| Sensitivity | 97.80% |
| Specificity | 99% |
| Accuracy | 98.50% |
| Cut-Off | Ref. Analytes |
Applications:
The Calprotectin and Lactoferrin Combo Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Calprotectin and lactoferrin in human fecal specimen which might be useful for the diagnosis of inflammatory gastrointestinal disorders.
Description:
Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8
and S100A9. The complex accounts for up to 60% of the soluble
protein content of the neutrophil cytosol. Calprotectin becomes
available in the intestinal lumen via leukocyte shedding, active
secretion, cell disturbance, and cell death.
This results in elevated faecal calprotectin levels, which can be
detected in the stool. Elevated faecal calprotectin levels
therefore indicate migration of neutrophils into the intestinal
mucosa, which occurs during intestinal inflammation.Faecal
calprotectin has been used to detect intestinal inflammation, and
can serve as a marker for inflammatory bowel diseases. Calprotectin
is useful as a marker, as it is resistant to enzymatic degradation,
and can be easily measured in faeces.
Lactoferrin is one of the components of the immune system of the
body; it has antimicrobial activity (bacteriocide, fungicide) and
is part of the innate defense, mainly at mucoses.In particular,
lactoferrin provides antibacterial activity to human infants.
Human lactoferrin, a neutrophil derived glycoprotein, can be
measured in feces and whole gut lavage as an indicator of
intestinal inflammation in both IBD and infectious gastroenteritis.
Recent studies have shown fecal lactoferrin (FL) as a sensitive
biomarker for pediatric IBD. In addition, this biomarker can serve
as an aid for guiding the diagnostic and therapeutic process for
both pediatric and adult IBD.
How to use?
Allow the test, specimen, buffer and/or controls to reach room
temperature (15-30°C) prior to testing.
1.To collect fecal specimens:
Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean,
dry specimen collection container to obtain maximum antigens (if
present). Best results will be obtained if the assay is performed
within 6 hours after collection. Specimen collected may be stored
for 3 days at 2-8℃ if not tested within 6 hours. For long term
storage, specimens should be kept below -20℃.
2.To process fecal specimens:
• For Solid Specimens:
Unscrew the cap of the specimen collection tube,then randomly stab
the specimen collection applicator into the fecal specimen in at
least 3 different sites to collect approximately 50 mg of feces
(equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
• For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then
transfer 2 drops (approximately 80 μL) into the specimen collection
tube containing the extraction buffer.
3.Tighten the cap onto the specimen collection tube, then shake the
specimen collection tube vigorously to mix the specimen and the
extraction buffer. Leave the tube alone for 2 minutes.
4.Bring the pouch to room temperature before opening it. Remove the
test cassette from the foil pouch and use it within one hour. Best
results will be obtained if the test is performed immediately after
opening the foil pouch.
5.Hold the specimen collection tube upright and open the cap onto
the specimen collection tube. Invert the specimen collection tube
and transfer 2 full drops of the extracted specimen (approximately
80 μL) to each specimen well of the test cassette, then start the
timer. Avoid trapping air bubbles in the specimen well (S). See
illustration below.
6.Read results at 5 minutes after dispensing the specimen. Do
notread results after 10 minutes.
7.Note: If the specimen does not migrate (presence of particles),
centrifuge the extracted specimens contained in the extraction
buffer vial. Collect 80 μL of supernatant, dispense into each
specimen well of a new test cassette and start afresh following the
instructions mentioned above.
INTERPRETATION OF RESULTS
(Please refer to the illustration above)
POSITIVE:* Two colored lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).
*NOTE: The intensity of the color in the test line region (T) will vary
depending on the concentration of Calprotectin and Lactoferrin
present in
the specimen. Therefore, any shade of color in the test line region
(T) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or
incorrect procedural techniques are the most likely reasons for
control line
failure. Review the procedure and repeat the test with a new test.
If the problem persists, discontinue using the test kit immediately
and contact your local distributor.
Order Information
| Cat. No. | Product | Specimen | Pack |
| OCAL-602 | Calprotectin Rapid Test Cassette | Feces | 10T |
| OLF-602 | Lactoferrin Rapid Test Cassette | Feces | 10 T |
| OIBD-625 | Calprotectin and Lactoferrin Combo Rapid Test Cassette | Feces | 10 T |
10T Calprotectin And Lactoferrin Test For Inflammatory Gastrointestinal Disorders
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