Sensitive Accurate Calprotectin FOB Rapid Test Cassette One Step Disposable
Brand Name:Citest
Certification:CE
Model Number:TCFC-625
Minimum Order Quantity:N/A
Delivery Time:2-4 Weeks
Place of Origin:-
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Product Details
Sensitive Accurate Calprotectin and FOB Rapid Test Cassette One Step Disposable for Diagnosis with fast reading
| Product features | Parameters |
| Principle | Chromatographic Immunoassay |
| Format | Cassette |
| Specimen | Feces |
| Certificate | CE |
| Reading Time | 5 minutes |
| Pack | 10 T |
| Storage Temperature | 2-30°C |
| Shelf Life | 2 Years |
| Cut-Off | Ref. Analytes |
Applications:
The Calprotectin and FOB Combo Rapid Test Cassette (Feces) is a
rapid chromatographic immunoassay for the qualitative detection of
calprotectin and/or Human Occult Blood in feces.
Description:
Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8
and S100A9. The complex accounts for up to 60% of the soluble
protein content of the neutrophil cytosol. Calprotectin becomes
available in the intestinal lumen via leukocyte shedding, active
secretion, cell disturbance, and cell death.
This results in elevated faecal calprotectin levels, which can be detected in the stool. Elevated faecal calprotectin levels therefore indicate migration of neutrophils into the intestinal mucosa, which occurs during intestinal inflammation. Faecal calprotectin has been used to detect intestinal inflammation, and can serve as a marker for inflammatory bowel diseases. Calprotectin is useful as a marker, as it is resistant to enzymatic degradation, and can be easily measured in faeces.
Many diseases can cause hidden blood in the feces. This is also
known as Fecal Occult Blood (FOB), Human Occult Blood, or Human
Hemoglobin. In the early stages, gastrointestinal problems such as
colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures
may not show any visible symptoms, only occult blood. Traditional
guaiac-based methods lack sensitivity and specificity, and also
have diet restrictions prior to testing.
How to use?
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1.To collect fecal specimens:
Collect sufficient quantity of feces (1-2mL or 1-2g) in a clean,
dry specimen collection container to obtain maximum antigens (if
present). Best results will be obtained if the assay is performed
within 6 hours after collection. Specimen collected may be stored
for 3 days at 2-8℃ if not tested within 6 hours. For long term
storage, specimens should be kept below -20℃.
2.To process fecal specimens:
• For Solid Specimens:
Unscrew the cap of the specimen collection tube,then randomly stab
the specimen collection applicator into the fecal specimen in at
least 3 different sites to collect approximately 50 mg of feces
(equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
• For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then
transfer 2 drops (approximately 80μL) into the specimen collection
tube containing the extraction buffer.
3.Tighten the cap onto the specimen collection tube, then shake the
specimen collection tube vigorously to mix the specimen and the
extraction buffer. Leave the tube alone for 2 minutes.
4.Bring the pouch to room temperature before opening it. Remove the
test cassette from the foil pouch and use it within one hour. Best
results will be obtained if the test is performed immediately after
opening the foil pouch.
5.Hold the specimen collection tube upright and open the cap onto
the specimen collection tube. Invert the specimen collection tube
and transfer 2 full drops of the extracted specimen (approximately
80μL) to the each specimen well (S) of the test cassette, then
start the timer. Avoid trapping air bubbles in the specimen well
(S). See illustration below.
6.Read results at 5 minutes after dispensing the specimen. Do not
read results after 10 minutes.
Note: If the specimen does not migrate (presence of particles),
centrifuge the extracted specimens contained in the extraction
buffer vial. Collect 80ul of supernatant, dispense into the
specimen well (S) of a new test cassette and start afresh following
the instructions mentioned above.
INTERPRETATION OF RESULTS
(Please refer to the illustration)
POSITIVE:* Two colored lines appear. One colored line should be in the control line region (C) and
another apparent colored line should be in the test line region
(T).
*NOTE: The intensity of the color in the test line region (T) will vary
depending on the concentration of Calprotectin and/or FOB present
in the specimen. Therefore, any shade of color in the test line
region (T) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques
are the most likely reasons for control line failure. Review the
procedure and repeat the test with a new test. If the problem
persists, discontinue using the test kit immediately and contact
your local distributor.
Order Information
| Cat. No. | Product Description | Specimen | Format | Kit Size | Cut-Off | Status |
| TCFC-625 | Calprotectin and FOB Combo Rapid Test Cassette | Feces | Cassette | 10 T | See Insert | CE |
Sensitive Accurate Calprotectin FOB Rapid Test Cassette One Step Disposable
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