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DAD Detector HPLC Instrument In Qualitative And Quantitative Analysis

DAD Detector HPLC Instrument In Qualitative And Quantitative Analysis

Brand Name:Wayeal
Certification:ISO, CE
Model Number:LC3100
Minimum Order Quantity:1
Delivery Time:20 days
Payment Terms:L/C, T/T
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Location: Hefei Anhui China
Address: No. 8, Wenqu Road, High-tech Zone, Hefei, Anhui
Supplier`s last login times: within 48 hours
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Product Details

DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Qualitative and Quantitative Analysis

Product Description


High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure. HPLCinstruments consist of a reservoir of mobilephase, a pump, an injector, a separation column, and adetector. Compounds are separated by injecting a sample mixtureonto the column. The different component in the mixture pass through thecolumn atdifferentiates due to differencesintheirpartition behavior between the mobile phase and thestationary phase. The mobile phase must be degassed to eliminate theformation of air bubbles.


Product Parameter


DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Qualitative and Quantitative Analysis
Physical Specification
Power Requirements220V,50HZ
Ambient Temperature4-40℃
Ambient Humidity20-85% relative humidity(non-condensing)
System
Qualitative repeatability(Retention time)RSD≤0.3%
Quantitative repeatability(Area)RSD≤0.6%
Pump
Flow Rate Range0.0001-10.000mL/min( increments :0.0001 mL/min)
Max Operating Pressure42MPa
Pressure Pulsation<0.1MPa( Test condition: 10MPa , at 1.0ml/min methanol)
Flow Accuracy±0.2%( Test condition: 10MPa , at 1.0ml/min methanol)
Flow PrecisionRSD≤0.06%( Test condition: 10MPa , at 1.0ml/min methanol)
Gradient Accuracy±1%
Column Oven
Temperature Setting Range5 °C ~ 85 °C
Temperature Stability≤0.2°C/h
Temperature Controlling Precision±0.1 °C
Autosampler
Injection Volume Precision

<0.25%RSD( full loop) ;

<0.5%RSD( partial loop) ;

<1%RSD( no waste)

Linearity>0.999
Cross Contamination(Carry Over)<0.01%
Sample Capacity120 Samples( 1.5ml vials)
Syringe250 μL( Standard, with 500μL,1000μL, 2500μL optionals)
Sample Loop20μL( Standard, with 50μL, 100μL, 200μL optional)

DAD Detector

Noise1.5×10-5Au
Drift≤1×10-3Au/h
LOD5.0×10-9g/mL (naphthalene/methanol)
Wavelength Range190-800nm
Wavelength Reproducibility≤±0.1 nm
Linearity≥1.0×104



Product Performance

1. Two optional modes: binary high pressure and quaternary low pressure


2. Wavelength scaning: the absorption spectrum can be scanned in the entire range of the light source.


3. A variety of detectors available, such as DAD, ELSD and FLD.


4. Dual-wavelength detection, two wavelengths canbe detected at the same time.


Product Application

Food, medicine, chemical industry, agriculture, etc.







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DAD Detector HPLC Instrument In Qualitative And Quantitative Analysis

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