Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized
Brand Name:Biokey
Certification:ISO 13485,CE
Model Number:BIK-QL-H003S
Minimum Order Quantity:We can produce liquid and lyophilized kits
Delivery Time:Depending on order quantity
Payment Terms:L/C, T/T, Western Union
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Real Time Diagnostics PCR Enterovirus 71 ( EV71 ) RT-PCR Detection Kit ( Lyophilized )
Intended use:
By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.
Test Principle:
The specific primers and specific fluorescent probes are designed
for the nucleic acid conserved region of EV71. The RT-PCR reaction
solution and real-time fluorescent quantitative PCR detection
technology is applied on the fluorescence quantitative PCR
instrument for realizes the rapid detection of EV71 through the
change of fluorescence signal. The detection system contains a
human internal control (IC) gene, which is used to monitor whether
the specimens and the process of nucleic acid extraction is
qualified.
Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophi...
Product components:
| No. | Components | BIK-QL-H003S |
| 1 | PCR Enzyme Mix (Lyophilized) | 48 tests/kit |
| 2 | EV71 Primer-Probe Mix | / |
| 3 | Enzyme Mix Buffer (5×) | / |
| 4 | Positive Control | 100 μL/tube |
| 5 | Negative Control | 100 μL/tube |
| 6 | RNase-free water | 1mL/tube |
| 7 | Paraffin oil | 1.5mL/tube |
Note: Do not mix the components from different batches for
detection. The Positive Control of EV71 and internal control were
constructed artificially, and they were not infectious.
Compatible instruments:
The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).
Reaction Mix Preparation:
Refer to one of the table below to prepare the reaction mix:
1×volume required | |
| Resuspended master mix | 14 μL |
| EV71 Primer & probe | 1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
PCR amplification condition:
PCR amplification according to the following procedure:
| Step | Cycle | Temperature | Time |
| 1 | 1 | 50℃ | 10 min |
| 2 | 1 | 94℃ | 3 min |
3 | 40 | 94℃ | 10 s |
| 60℃ | 30 s (Collection of fluorescence) |
Select the detection channels as following:
a) Select FAM channels to detect EV71.
b) Select HEX/VIC channel to detect internal control.
Reaction Mix Preparation:
Refer to one of the table below to prepare the reaction mix:
| 1×volume required | |
| Resuspended master mix | 14 μL |
| EV71 Primer & probe | 1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
Product picture:
Operation details please check from IFU
Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized
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