Diagnostic Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit

Diagnostic / Laboratory virus Fluorescence PCR kit Siniperca chuatsi rhabdovirus Nucleic Acid PCR Kit

Intended use:

This kit is suitable for auxiliary diagnosis of Siniperca chuatsi rhabdovirus (SCRV) infection in animal tissue, feed, feces and other samples.

Principle:

This kit to choose a conservative design specific gene probe, the probe can with primer amplification area in the middle of Siniperca chuatsi rhabdovirus (SCRV) specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Siniperca chuatsi rhabdovirus (SCRV) nucleic acid in the completely closed reaction system was realized by fluorescence PCR instrument.

Main components:

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM was selected for detection channel

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

Product performance index:

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: The detection limit of this kit is 10 copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

Quality control:

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

Operation details please check from IFU!

Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team.

The company's core team has been in the field of IVD research and entrepreneurship for more than 10 years, and has mastered a wealth of molecular diagnostic POCT industrial application technology, SmartTRF immune detection technology, RT qPCR nucleic acid detection technology and independent research and development capabilities of instruments.

It has a number of independent core technologies in the fields of automation engineering, micro-precision manufacturing, immunofluorescence, nucleic acid detection and gene sequencing. Since its establishment, the company has applied for more than 60 patents and currently has 17 authorized patents. The company has two R&D centers for diagnostic reagents and instruments and equipment, totaling more than 500 square meters; it has established more than 2,000 square meters of GMP workshops and equipment production plants; it has SmartTRF new immune detection technology, nucleic acid detection technology (POCT) and three scientific services.