Product Details
Feline Calici virus (FCV) PCR kits, real time quantiative PCR
Fluorescent Probe Kits
Intended use:
This kit is used for the specific detection of feline calicivirus
(FCV) in cat eye, nose and mouth secretions.
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Choose cat goblet
virus gene conservative design specific probe, the probe can with
primer amplification area in the middle of a specific binding DNA
template, in the process of PCR extension, the circumscribed Taq
polymerase enzymes from the 5 'end of fluorescent groups on the
probe will be cut down, make it free in the reaction system,
thereby quenching groups out of the 3' end, glow, The nucleic acid
of cat calicivirus was detected by fluorescence PCR in a completely
closed reaction system.
Biokey Pet PCR testing solution.pdf
| Component | Specification |
| PCR reaction liquid | 8 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Storage: -20±5℃
After sample preparation, proceeding to PCR thermal cycling below
steps:
| step | cycle | termperature | time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s |
Details please check IFU!
Company Profile
Bio key Health is a high-tech enterprise with integrating R&D,
production and sales&marketing in Guangzhou. Since 2003. Bio
Key was founded by the "Thousand Talents Program" entrepreneurial
team and the overseas senior automation equipment development
engineer team.
The company's core team has been in the field of IVD research and
entrepreneurship for more than 10 years, and has mastered a wealth
of molecular diagnostic POCT industrial application technology,
SmartTRF immune detection technology, RT qPCR nucleic acid
detection technology and independent research and development
capabilities of instruments.
It has a number of independent core technologies in the fields of
automation engineering, micro-precision manufacturing,
immunofluorescence, nucleic acid detection and gene sequencing.
Since its establishment, the company has applied for more than 60
patents and currently has 17 authorized patents. The company has
two R&D centers for diagnostic reagents and instruments and
equipment, totaling more than 500 square meters; it has established
more than 2,000 square meters of GMP workshops and equipment
production plants; it has SmartTRF new immune detection technology,
nucleic acid detection technology (POCT) and three scientific
services.