Pirlimycin ELISA Test Kit / Milk Analysis Kit Competitive Enzyme Immunoassay

Pirlimycin ELISA Test Kit

Product Description

REAGEN^™ Pirlimycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of pirlimycin in milk. Pirlimycin is a lincosamide antibiotic. Under the tradename Pirsue, it is used inthe treatment of mastitis in cattle. Pirlimycin is active against gram-positive bacteria, specifically Staphylococcus aureus and coagulase negative species of Staphylococcus and Streptococcus. It is bacteriostatic and acts by inhibiting bacterial protein synthesis via binding with the 50S subunit of the ribosome.

Pirlimycin ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect pirlimycin in various sample types and to satisfy customer concerns about food safety.

The unique features of the kit are:

1.Rapid (10 - 40 minutes), cost-effective extraction methods offering high recovery rates

2.High sensitivity (5.0 ng/g or ppb)

3.Quick ELISA assay (less than 1.5 hours regardless of number of samples)

4.High reproducibility

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The Pirlimycin antibody has been coated in the plate wells. During the analysis, sample and Pirlimycin -horseradish peroxidase (Pirlimycin -HRP) conjugate are added to the wells for incubation. If the Pirlimycin residue is present in the sample, it will compete for the Pirlimycin antibody, thereby preventing the Pirlimycin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Pirlimycin residue concentration in the sample.

Pirlimycin ELISA Test Kit