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Quinolone rapid test dipstick
Brief:
This product is used for testing Quinolone residue in milk
sample(pure milk and fresh milk) and goat’s milk qualitatively, the
whole test procedure only need 6min, easy to operate with high
sensitivity.
Principle:
The Quinolone rapid test dipstick is based on competitive
inhibition immuno-chromatographic principle. In the flow process,
Quinolone in the sample combined with Quinolone specific colloidal
gold-labeled monoclonal antibody, Inhibit the combination between
antibody and Quinolone -BSA conjugate on Test line of NC membrane,
lead to the color change of Test line.
When the sample has no Quinolone residue or concentration lower
than detection limit, T line is darker than C line. when the
concentration is equal to or higher than detection limit, T line is
lighter than C line obviously or T line is invisible or T line has
same color with C line. No matter whether there is Quinolone
residue in sample, C line will appear, it means the test is valid.
Operation procedures:
1 Read the instruction carefully before use. Return test dipsticks
and sample into room temperature.
2 Take bottles needed from the kit package, take out required
micro-wells and dipsticks, making proper marks. Please use these
test dipsticks within 1h. Seal the cap of the bottles, avoid
moisture.
3 Take 200ul of the test samples into the micro-wells, then
repeatedly absorb for 5 times, mix the sample with the reagent in
the micro-wells completely until no solid judge by eyes (this is a
very important step).
4 Incubate for 3min with Incubator(40±2℃), then insert the test
dipsticks into the micro-wells with the "MAX" end fully dipped in
to the mixture solution.
5 Insert the test dipsticks into micro-wells for 3min then read the
result, it is invalid in other time.
Test Result Interpretation:
Color depth comparison of T line and C line | Result judgment | Result analysis |
T line > C line | Negative | Quinolone residue is lower than detection limit in sample |
T line = C line | Weak positive | Quinolone residue is equal to detection limit in sample |
T line < C line or T line is invisible | Positive | Quinolone residue is higher than detection limit in sample |
C line is invisible | Invalid | The test dipstick is invalid, test again |
Results picture show:
Precautions:
1) The test dipsticks can be used only once at room temperature, do
not use test dipsticks out of expiry date.
2) Please seal the bottle after taking out required test dipsticks.
If can not use all 8 micro-wells at once, cup the rest and put it
back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test
dipsticks, avoid sunlight and fan blowing directly.
4) Milk samples should be fully liquid without any agglomeration,
sour and precipitation.
5) Use the test dipsticks testing again for positive results.
6) Please contact the supplier for any questions.
7) The result is for reference only, for confirmation, please refer
to the relevant national standard method.
Specificity:
Test 500ppb of Tetracyclines, Chloramphenicol, Sulfonamides etc.,
the result is Negative.
Storage and expiry date:
Storage: Store at 2-8 ℃ in sealed, dry, and dark place,no frozen.
Expiry date: 12 months; date of production is on box.
Detection limit in milk (ug/kg or ppb)
Enrofloxacin | 1-2 | Flumequine | 1-2 |
Ciprofloxacin | 1-2 | Enoxacin | 2-4 |
Norfloxacin | 1-2 | Lomefloxacin | 2-3 |
Pefloxaein | 1-2 | Marbofloxacin | 5-6 |
Sarafloxacin | 1-2 | Nadifloxacin | 5-6 |
Ofloxacin | 1-2 | Difloxacin | 2-3 |
Danofloxacin | 3-5 | Cinoxacin | 5-6 |
Oxolinic Acid | 1-2 |