30ul Automated Nucleic Acid Extractor
Brand Name:HAIYING
Certification:CE certificate
Model Number:AU1001-96
Minimum Order Quantity:1 set
Delivery Time:After-sales Service Provided: Online support, Video technical support, Free spare parts
Payment Terms:T/T
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Product Details
High Capacity 96 Nucleic Acid Purification System Automated Nucleic
Acid Extractor
Nucleic Acid Extractor Introductiion
High Capacity Wide Range Of Applications Automated Nucleic Acid
Extractor is based on the magnetic bead method to extract DNA in
the sample. It uses specially designed nanomagnetic beads to absorb
DNA under different conditions. By adsorbing, washing and eluting
the DNA in the sample Wait for a series of operation procedures to
obtain purified DNA template. The main extraction process is
divided into the following three steps:
(1) Sample processing: processing according to different sample
types, cutting or drawing a small amount of sample, and adding it
to a 1.5ml Ep tube;
(2) Lysis release: Add the lysis solution to the above-treated
sample, mix it evenly (vortex and shake), and then place it in a
constant temperature incubator at 65°C for 30-60 minutes;
(3) Separation and purification: add the incubated sample to the
single sample or whole plate extraction kit of the instrument, and
run the edited automatic extraction program to obtain the purified
DNA template.
Among them, the single-sample extraction kit can extract any 1~8
samples, the whole-plate extraction kit can extract 16 samples, and
a maximum of 32 samples can be extracted in one run (two plates),
providing a flexible and convenient extraction scheme and saving
reagent consumables costs . The instrument uses the cooperation of
the magnetic rod and the magnetic rod cover to achieve the
adsorption and release of the magnetic beads and complete the
sample DNA extraction process.
Nucleic Acid Extractor Parameters
| Model | AU1001-96 |
| Brand Name | HAIYING |
| Instrument classification | Class I |
| After-sales Service Provided | Online support |
| magnetic head | 96 |
Nucleic Acid Extractor Application
High Capacity Wide Range Of Applications Automated Nucleic Acid
Extractor is generally divided into two types: suction method and
magnetic rod method:
1. The suction method, also called the pipetting method, is to
extract nucleic acid by fixing magnetic beads and transferring
liquid. Generally, the transfer is realized by controlling the
mechanical arm through the operating system. The extraction process
is as follows:
1) Lysis: Add the lysis solution to the sample, realize the mixing
and full reaction of the reaction solution through mechanical
movement and heating, cell lysis, and release of nucleic acid.
2) Adsorption: Add magnetic beads to the sample lysing solution and
mix them thoroughly. The magnetic beads have a strong affinity for
nucleic acids under high salt and low pH values to adsorb nucleic
acids. Under the action of an external magnetic field, the magnetic
beads are separated from the solution. , Use the tip to remove the
liquid and discard it to the waste tank, and discard the tip.
3) Washing: Remove the external magnetic field, replace with a new
tip, add washing buffer solution, mix thoroughly to remove
impurities, and remove the liquid under the action of an external
magnetic field.
4) Elution: Remove the external magnetic field, replace with a new
tip, add elution buffer, mix thoroughly, and the bound nucleic acid
is separated from the magnetic beads to obtain purified nucleic
acid.
2. The magnetic rod method realizes the separation of nucleic acids
by fixing the liquid and transferring the magnetic beads. The
principle and process are the same as the suction method, but the
difference is the method of separating the magnetic beads and the
liquid. The magnetic rod method is to separate the magnetic beads
from the waste liquid through the adsorption of the magnetic beads
on the magnetic rod, and put them into the next liquid to realize
the extraction of nucleic acid.
Nucleic Acid Extractor Details
30ul Automated Nucleic Acid Extractor
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