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Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Kit composition (WLRE8208KIT 48T/Pack) | |
Composition | Content |
E buffer | 1 mL×2 Tubes |
B buffer | 150 μL×1Tube |
Positive control template | 100 μL×1Tube |
Reagents | 48T |
Product features and advantages | |
Features | Advantages |
High sensitivity | 1 copies/ml |
Strong specificity | Specificity is superior to PCR |
Short reaction time | 20min |
Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the results Design of primers and probe is simple |
Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light. The freeze-dried form has a long storage time and is convenient for transportation |
Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument; Applicable to our WL-16-III and other isothermal fluorescence detector |
Application | |
Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
Indoor |
Operation procedure and process: |
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
No. | TT value | Template concentration | Result determination |
1 | 4.2 | 10-1 | Positive |
2 | 5.5 | 10-2 | Positive |
3 | 10.2 | 10-3 | Positive |
4 | 17.0 | 10-4 | Positive |
5 | 12.0 | 10-5 | Positive |
6 | - | 10-6 | Negative |
7 | - | 10-7 | Negative |
8 | Negative control | Negative |
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