Pathological Acid Fast Stain Reagents Phenol Free Fuchsin Staining Method

Pathological Acid Fast Stain (Phenol-free fuchsin staining method) Baso Clear Colourful Stains

Testing Principle

Acid-fast bacteria, such as Mycobacterium (e.g., tuberculosis bacilli), are difficult to stain due to a layer of lipid or lipoid membrane on their surface. However, once stained, they resist decolorization even when treated with acid-alcohol decolorizing solution. This characteristic is utilized by first staining with an enhanced staining solution, then treating with acid-alcohol decolorizer, and finally counterstaining. As a result, acid-fast bacteria retain the initial stain (red) and are easily identified. This product improves upon the traditional Ziehl-Neelsen acid-fast staining method by modifying the carbol fuchsin solution to exclude phenol (also known as carbolic acid), while still ensuring that acid-fast bacteria are vividly stained red. The counterstain uses malachite green, creating a sharp contrast between the red color of the acid-fast bacteria and the green background.

Stain Results
Against a light green background, acid-fast bacteria (e.g., tuberculosis bacilli) appear red, while other bacteria and cells appear green.

Mycobacterium tuberculosis is mostly rod-shaped and slightly curved. The width of the bacteria ranges from 0.3 to 0.6 µm, and the length varies between 0.5 to 8 µm, with most falling within 1.5 to 3.5 µm. A small number of longer bacteria may exhibit a spiral shape. In fresh specimens containing a high concentration of Mycobacterium tuberculosis, acid-fast staining and observation under a 100x oil immersion lens can reveal both individually dispersed bacteria and clusters or branching arrangements of bacteria.

Packaging Specification

Wuhan Jinhong Biotech Development Co., Ltd is the sales center of Baso products and also as an applicant and manufacturer of CE certificate of Plasma Freezing System with Intelligent Temperature Control. Wuhan Jinhong, since 1993, is a very professional team with a sales network covering 31 provinces, municipalities and autonomous regions across the country. At the same time, it has long-term cooperation with agents, distributors and companies to ensure customers enjoy high efficiency, high quality post-sales service.

Baso Group commit to continuous innovation and quality improvement, and constant effort to reach the level of the world's first. We hope to share the good quality to every customer around the world.