High Iron Diamine -Alcian Blue Stain (HID-AB) Baso Clear Colourful Stains

Testing Principle
N,N-Dimethyl-m-phenylenediamine dihydrochloride and N,N-dimethyl-p-phenylenediamine dihydrochloride are both amine salts that dissociate to carry positive charges. The diamine salts combine with sulfated acidic mucosubstances to form colored complexes. This reaction is slow and requires ferric chloride (FeCl₃) as a catalyst. On one hand, FeCl₃ oxidizes the diamine salts to form brown-black cationic chromogens, thereby accelerating the staining process. On the other hand, it lowers the pH of the staining solution to 1.4. At this pH, carboxyl groups on the tissue sections cannot bind to the diamine salts, and only sulfate groups react with the diamine salts to form purple-brown to brown-black complexes. Subsequently, Alcian Blue (pH 2.5) stains carboxylated sialomucins blue. In this way, the two main groups of acidic mucosubstances are distinctly visualized.

Stain Results

Acidic mucopolysaccharides appear blue, while other tissue cells appear light red.

Packaging Specification

Wuhan Jinhong Biotech Development Co., Ltd is the sales center of Baso products and also as an applicant and manufacturer of CE certificate of Plasma Freezing System with Intelligent Temperature Control. Wuhan Jinhong, since 1993, is a very professional team with a sales network covering 31 provinces, municipalities and autonomous regions across the country. At the same time, it has long-term cooperation with agents, distributors and companies to ensure customers enjoy high efficiency, high quality post-sales service.

Baso Group commit to continuous innovation and quality improvement, and constant effort to reach the level of the world's first. We hope to share the good quality to every customer around the world.