Optimus Hotstart Taq DNA Polymerase PCR Reagent Enzyme P1041

Optimus^TM Hotstart Taq DNA Polymerase

#P1041, 250U

Optimus Hotstart Taq DNA Polymerase Pcr Reagent High Specificity P1041 250U

Concentration: 5U/μl

Contents:

Store at -20°C

For research use only.

In total 3 vials.

Description

Optimus^TM Hotstart Taq DNA Polymerase is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 1min/kb (simple template up to 20s/kb) separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The product of Hotstart Taq has overhanged dA at 3’-end. Optimus^TM Hotstart Taq DNA Polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.

Optimus^TM Hotstart Taq DNA polymerase is an innovative and useful product.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

200mM Tris-HCl, 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 50% (V/V) glycerol, 0.5% Triton X -100

10× Hotstart Buffer with Mg2+

50mM KCl, 100mM Tris-HCl, 200mM NH₄Cl, 20mM MgCl₂.

Applications

• High-specificity amplification & multiplex PCR: hot-start polymerase with chemical modification

• Thermostable: half-life over 40 min at 95°C incubation

• Generates 3'-dA overhangs PCR products

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

GDSBio's international resource links and multi-dimensional biological research methods help it stand at the forefront of the industry. To interpret the world with high-end technology, insight into the biological mystery.

ISO 13485 certificate

ISO 9001 certificate