Home /Other Switches /

New Chinese Manufacturers Cost-Effective Sequencing Library Building Kit

New Chinese Manufacturers Cost-Effective Sequencing Library Building Kit

Brand Name:GDSBio
Certification:ISO9001, ISO13485
Model Number:KM001-A, KM001-B
Minimum Order Quantity:1 kit
Delivery Time:8 work days
Payment Terms:L/C, D/A, D/P, T/T, Western Union, MoneyGram
Contact Now

Add to Cart

Verified Supplier
Location: Guangzhou Guangdong China
Address: Room 305, Building A, No. 179, Guangpu East Road, Huangpu District, Guangzhou, Guangdong
Supplier`s last login times: within 1 hours
Shipping
lt's easy to get a shipping quote! Just click the button below and complete the short form.
Get Shipping Quote
Product Details Company Profile
Product Details

Fast DNA Library Prep Kit for MGI

【Product Name】

Fast DNA Library Prep Kit for MGI

【Cat. No./Spec.】

KM001-A/24 rxns; KM001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

ApplicationSample TypeRecommended Amount
Whole genome sequencingHigh quality complex genomes50ng-1μg
Target capture sequencing of whole exomeHigh quality complex genomes10ng-1μg
Target capture sequencing of whole genomeFFPE DNA≥50ng
Target capture sequencing of whole genomecfDNA/ctDNA≥100pg
Whole genome sequencingMicrobial genome1ng-1μg
ChIP-SeqChIP DNA≥100pg
Targeted sequencingAmplicon≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component24 rxns96 rxns
End Repair & A-Tailing Mix480 μl4×480 μl
Fast DNA Ligase120 μl2×240 μl
Fast Ligation Buffer600 μl4×600 μl
2× HIFI Library PCR Master Mix600 μl4×600 μl
Primer Mix for MGI*120 μl480 μl

* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers, the primer sequence is as follows:

5’-TGTGAGCCAAGGAGTTG-3’

5’-GAACGACATGGCTACGA-3’

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

GDSBio Is a high-tech enterprise focusing on research and development, production and sales of high-quality life science products. The company has a complete product line, with PCR technology as the core, focusing on general PCR, fluorescence quantitative PCR, NGS storage, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.


China New Chinese Manufacturers Cost-Effective Sequencing Library Building Kit supplier

New Chinese Manufacturers Cost-Effective Sequencing Library Building Kit

Inquiry Cart 0