GDSBio Taq DNA Polymerase For PCR Master Mix

Taq DNA Polymerase

#P1015, 18,000U

Concentration: 5U/μl

Contents:

Store at -20°C

For research use only.

In total 108 vials.

Description

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH 8.0), 100mM KCl, 3.2mM MgCl_2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.

10× PCR Buffer without Mg2+

100mM Tris-HCl (pH 8.8), 500mM KCl, 1% Triton X-100.

Applications

• PCR amplification of DNA fragments as long as 5 kb

• DNA labeling

• DNA sequencing

• PCR for cloning

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

GDSBio's international resource links and multi-dimensional biological research methods help it stand at the forefront of the industry. To interpret the world with high-end technology, insight into the biological mystery.

ISO 13485 certificate

ISO 9001 certificate