Animal Serum Or Plasma IgG ELISA Toxoplasma Rapid Test Kit For Pet 96 Wells/Kit

Toxoplasma Gondii IgG Antibody ELISA Kit for Pet 96 Wells/Kit GMP ISO9001

1. Principle

This kit use indirect ELISA method to test specific Toxoplasma gondii antibody in animal serum or plasma, it can be used as animal toxoplasmosis secondary inspection. If there is Toxoplasma gondii antibody in the sample, it will combine with coated Toxoplasma antigen on plate and SPA enzyme conjugate on the second step. The color reaction will reflect whether there is toxoplasma antibody in the sample.

2. Reagents and contents

3. Materialrequired notprovided

1) Microplate Reader with 450nm and 630nm
2) 37 ℃thermostatic device
3) Micropipettes, adjustable.

4. Samplerequirement

1) This kit is used for onlydog, cat, rabbit and monkeyetc. serum and plasma
2) To get the best test result, avoid using sample with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3) The serum sample store at 2-8 ℃for 3 days, if for long term, it should be kept at -20℃or lower, avoid repeated freezing and thawing.

5. Test procedure

1) Washing Solution preparation: Dilute 10X Washing solution with distilled water or deionized water at 1:10 (for example: take 100ml 10X Washing solution, add 900ml distilled water or deionized water, mix),mix it evenly to get washing solution.
2) Sample diluent and adding sample: Dilute serum with Sample diluent at 1:100, Add 100μl diluted serum sample into all sample wells; If using Serum diluent plate, the dilution method is like following:
Dilute at 1:100: Firstly add 50ul sample diluent into the coated sample wells. On Serum diluent plate, add 245ul Sample diluent, then add 5ul serum, use Pipette to mix it evenly, then take 50ul
Into coated sample wells for react. (Note: Serum diluent plate is disposable, can not repeat use.)
3) Add controls: Positive control and negative control do not need dilute, add directly. Set 2 blank controls, only add sample diluent,100ul/well. Set 2 wells for negative control, only add 100ul negative control; 2 wells for positive control, only add 100ul positive control. Cover andIncubate in dark at 37 ℃ for30 minutes.
4) Washing plate: Discard the liquid of the well, add washing solution into each well fully(250ul/well if using washing machine) for 3 times, incubate for 1 min each time, at last time flap to dry with the absorbent paper.
5) Adding Enzyme conjugate: Add 50ul Enzyme Conjugate into all wells (except blank well) andincubate at 37℃ in dark for30 minutes. Discard the contents of the wells and wash 3 times as described in step 3.
6) Adding substrates: according to quantity needed, Add equal volume of Substrate A and Substrate B, mix evenly. Add 100ul into all well, incubate at 37℃ in dark for 10 minutes.
7) Add 50ul Stop solution into all wells to stop reaction. Set zero at blank control, read the OD value at ELISA reader 450nm (630nm as a reference).

6. Resultsinterpretation

1) Cut-off value(COV)=Average OD value of negative control x 2.1 (calculate as 0.08 when the OD value of negative control is less than 0.08)
2) Validation judgement:
OD value of negative control≤0.15 (If＞0.15, invalid);
OD value of positive control≥0.50 (If＜0.50, invalid);
3) Results
Sample OD value ≥ COV, the result is Positive;
Sample OD value < COV, the result is Negative;
4) Limitation: This kit is only for Screening, not basis for diagnosis.

7. Notes

1) MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at 2~8 ℃.
2) Before adding reagent, gently shake dropping bottle and mix even the reagent.
3 When incubation, must cover the plate with adhesive foil, do not repeat use the adhesive foil.
4) Screw down the cap after use, don't mix caps between different bottles and don't mix components between different kits
5) The test kit and any samples should be regarded as the source of infection to properly handle. Stop solution is corrosive, be careful to use.

8. Storage and expire date

Store at 2~8℃ in dark, no frozen, expiry date: 12 months.

Pathogen and its life history

-The pathogen of toxoplasmosis is Toxoplasma gondii, or Toxoplasma gondii for short. Its entire development process requires two hosts. The cat is the final host of Toxoplasma gondii. In the epithelial cells of the small intestine of the cat, it carries out fission proliferation and gametogenesis similar to the development of coccidia, and finally forms an oocyst, which is discharged with the cat feces. In the external environment, the oocyst develops into an infectious oocyst containing two sporangia through spore proliferation.

- Toxoplasma gondii has no strict choice of intermediate host. It is known that more than 200 species of animals, including mammals, birds, fish, reptiles and humans, can serve as its intermediate host. Cats can also serve as intermediate hosts of Toxoplasma gondii. In the intermediate host, Toxoplasma gondii can reproduce asexually in the nucleated cells of various tissues and organs of the body.

- Animals are infected by eating infected oocysts in cat feces or meat, viscera, exudates, excreta and milk of intermediate hosts containing tachyzoites or cysts of Toxoplasma gondii. Tachyzoites can also infect the fetus through skin, mucous membrane and placenta.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.

Shenzhen Wensidun Technology Co., Ltd. was established in 2018 and is located in Longgang District, Shenzhen, Guangdong Province, China. We have in-depth cooperation with Green Spring, which has 20 years of production and R&D experience.

We have more than 2000 square meters of GMP clean production workshop and more than 1000 square meters of R&D center in Shenzhen. Products cover over 100 varieties of food safety test kits, animal diseases diagnostic kits, in vitro diagnostic reagents, genetic antibody antigen preparation and form a complete set of testing instrument.Currently,our food safety test kit and animal disease diagnostic kit are widely used in many governments,enterprises and research institutes.Our products are sold to more than 40 countries and areas.

We have ISO9001, ISO13485 quality management system certification, GMP certificate. SARS-CoV-2 Antigen Rapid Test Kit has been awarded CE certification, German BfArM approved self-test and professional test, PEI certification, EU general and mutual recognition list, and multinational white list. Ranked No. 1 in German authoritative medical magazine Daz and No. 1 in Hong Kong sales.

Enterprise mission: Caring for health and benefiting the society. Focus on products and lead the industry.

Core values: Inheriting innovation, improving quality, honesty and law-abiding, and win-win cooperation.

Our vision is to become a leading company in the biodiagnostic reagent industry, providing high-quality, convenient and reliable services to the world.