Bird Flu Subtype H9 Antibody ELISA Avian Influenza Rapid Test Kit 96 Wells/Kit

Bird Flu Subtype H9 Antibody ELISA Test Kit for Poultry 96 Wells/Kit

1. Usage

This kit is used to detect Bird Flu subtype H9 antibody in chicken serum, to assess antibody condition by Bird Flu subtype H9 vaccine in chicken farm and assist diagnosis of serological infected chicken.

2. Principle

The Bird Flu subtype H9 antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

3. Reagents

4. Materials required but not provided

1) Micropipettors and disposable tips: 0.5μL~10μL,10μL~100μL,100μL~1000μL
2) 37 ℃ Incubator
3) 96 wells microplate reader
4) Distilled water or deionied water
5) Bottle or microplate washing machine

5. Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.

6. Preparation of washing buffer

Return washing solution to room temperature before use, if there is salty crystals, shake to make the crystals dissolve, then use distilled water or deionized water to dilute it at 10 times. The diluted washing solution can store for 1 week at 4 ℃.

7. Sample dilution

At serum dilution plate, dilute serum at 1:100 with sample dilution (for example: 495μL sample dilution + 5μL serum)
Notice: Negative control and Positive control do not need dilute. Exchange tip after taking sample every time, record the situation of the sample on plate accurately. Shake the sample evenly before adding it.

8. Notes

1) All reagents should be adjusted to the room temperature and shake evenly before using, store at 2-8 ℃after using
2) Do not exchange the reagents from the kits of different lot numbers to use. Avoid reagent pollution when using.
3) Substrate and stop solution may have excitant to skin and eyes, pay attention when using.
4) Do not expose TMB (Substrate B) to light and avoid it contact with antioxidants.
5) The wells should avoid damp or touching water after unsealing (Put the un-using microplate back to bag with dehydrator in 2~8 ℃soon )
6) Deal all waste reasonable before dumping to avoid pollution.
7) Strictly adhere to instruction to get best result. All procedure including pipetting, timing and washing etc. must be accurate.
8) Serum dilution plate is disposable, do not use for second time; the MAX volume of it is 300μL/well.

9. ELISA procedure

1) Take pre-coated microplate (Can unseal for several time use as per sample quantity), add 100μL diluted serum to sample wells, meanwhile set 1 well for Negative control, 2 wells for Positive control separately. Add 100 μL Negative/Positive control to its wells. Shake softly (do not spill), cover and incubate at 37℃ for 30 min.
2) Pour the liquid out of the wells, add 250μL diluted washing solution to each well, pour out. Repeat 5 times, then pat to dry on absorbent paper.
3) Add 100 μL Enzyme Conjugate to each well, cover andincubate at 37℃ for 30 min.
4) Repeat the step 2(washing). Remember pat to dry on absorbent paper at last.
5) Add 100μL substrate solution to each well, mix properly,react for 10 min at 37℃ in dark.
6) Add 50 μL stop solution in each well, and measure the result within 10 min.

10. Results

Read the OD value with microplate-reader at 450nm (630nm as reference).
For the test to be valid:
OD value of Negative control (N) < 0.15, meanwhile OD value of Positive control (P)〉0.4.
Calculation method:
OD value of samples/ Average OD value of Positive control = S/P value
Result judge:
S/P≥0.25, Positive.
S/P < 0.25, Negative;

Specifications: 96 or 96*2 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.

Shenzhen Wensidun Technology Co., Ltd. was established in 2018 and is located in Longgang District, Shenzhen, Guangdong Province, China. We have in-depth cooperation with Green Spring, which has 20 years of production and R&D experience.

We have more than 2000 square meters of GMP clean production workshop and more than 1000 square meters of R&D center in Shenzhen. Products cover over 100 varieties of food safety test kits, animal diseases diagnostic kits, in vitro diagnostic reagents, genetic antibody antigen preparation and form a complete set of testing instrument.Currently,our food safety test kit and animal disease diagnostic kit are widely used in many governments,enterprises and research institutes.Our products are sold to more than 40 countries and areas.

We have ISO9001, ISO13485 quality management system certification, GMP certificate. SARS-CoV-2 Antigen Rapid Test Kit has been awarded CE certification, German BfArM approved self-test and professional test, PEI certification, EU general and mutual recognition list, and multinational white list. Ranked No. 1 in German authoritative medical magazine Daz and No. 1 in Hong Kong sales.

Enterprise mission: Caring for health and benefiting the society. Focus on products and lead the industry.

Core values: Inheriting innovation, improving quality, honesty and law-abiding, and win-win cooperation.

Our vision is to become a leading company in the biodiagnostic reagent industry, providing high-quality, convenient and reliable services to the world.