Avian Infectious Bronchitis Virus IBV Bird Flu Test Kit Antibody For Poultry 192 Wells/Kit

Infectious Bronchitis Virus (IBV) Antibody ELISA Kit for Poultry 192 Wells/Kit

1. Usage

This kit is used to detect Infectious Bronchitis Virus (IBV) antibody in chicken serum, to assess antibody condition by Infectious Bronchitis vaccine in chicken farm and assist diagnosis ofserological infected chicken.

2. Principle

The Infectious Bronchitis Virus (IBV) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

3. Reagents

4. Materials required but not provided

1) Micropipettors and disposable tips: 0.5μL~10μL,10μL~100μL,100μL~1000μL
2) 37℃Incubator
3) Measuringcylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine

5. Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.

6. Preparation of washing buffer

Return washing solution to room temperature before use, if there is salty crystals, shake to make the crystalsdissolve, then use distilled water or deionized water to dilute it at 10 times. The diluted washing solution can store for 1 week at 4℃.

7. Sample dilution

At serum dilution plate, dilute serum at 1:101 with sample dilution (for example: 200μL sampledilution + 2μL serum)
Notice:Negative control and Positive control do not need dilute. Exchange tip after taking sample every time, record the situation of the sample on plate accurately. Shake the sample evenly before adding it.

8. Notes

1) All reagents should be adjusted to the room temperatureand shake evenlybefore using,store at 2-8℃after using
2) Do not exchange the reagents from the kits of different lot numbers to use. Avoid reagent pollution when using.
3) Substrate and stop solution may have excitant to skin and eyes, pay attention when using.
4) Do not expose TMB (Substrate B) to light and avoid it contact with antioxidants.
5) The wells should avoid damp or touching water after unsealing (Put the un-using microplate back to bag with dehydrator in 2~8℃soon )
6) Deal all waste reasonable before dumping to avoid pollution.
7) Strictly adhere to instruction to get best result. All procedure includingpipetting, timing and washing etc. must be accurate.
8) Serum dilution plate is disposable, do not use for second time; the MAX volume of it is 300μL/well.

9. ELISA procedure

1) Take pre-coated microplate (Can unseal for several time use as per sample quantity), add 100μL diluted serum to a well, meanwhile set 1 wells forNegative control, Positivecontrol and blank control wells separately. Add 100μL Negative/Positivecontrol to its wells, only add 100μL sample dilution in the blank control wells.Shake softly (do not spill),
2) Cover and incubate at 37℃ for 30 min.
3) Pour the liquid out of the wells, add about 350 μL diluted washing solution to each well fully, static for1 min, pour out. Repeat3 times, then pat to dry on absorbent paper.
4) Add 100 μLEnzyme Conjugate to each well,coverandincubate at 37℃ for 30 min.
5) Repeatthestep3(washing). Rememberpat to dry on absorbent paper at last.
6) Add 50 μL substrate A, then substrateB (50 μL) to each well, mix properly,cover andreact for 10 min at 37℃ in dark.
7) Add 50 μL stop solution in each well, and measure the result within 10 min.

10. Results

Set zero for the blank well, and test theA450nm(630 nm as a reference)value on the microplate-reader. The conditions for the test to be tenable are that the positive control wells’A450nm value isgreater than or equal to 0.5, and the negative control wells’A450nm value isless than0.15. If the test is invalid, the operation is in doubt, retest and observe all the reagents carefully.
If the sample’s A450 value is greater than 0.15+absorbance of negative control mean, it is judged to be positive; and if less than 0.15+absorbance of negative control mean,negative. Ifabsorbance of negative control mean is less than 0.05, calculate as 0.05

Specifications: 96 wells/kit or 96*2 wells/kit
Expiry date:12months.
Storage: Storing at 2-8℃, in the dark.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.

Shenzhen Wensidun Technology Co., Ltd. was established in 2018 and is located in Longgang District, Shenzhen, Guangdong Province, China. We have in-depth cooperation with Green Spring, which has 20 years of production and R&D experience.

We have more than 2000 square meters of GMP clean production workshop and more than 1000 square meters of R&D center in Shenzhen. Products cover over 100 varieties of food safety test kits, animal diseases diagnostic kits, in vitro diagnostic reagents, genetic antibody antigen preparation and form a complete set of testing instrument.Currently,our food safety test kit and animal disease diagnostic kit are widely used in many governments,enterprises and research institutes.Our products are sold to more than 40 countries and areas.

We have ISO9001, ISO13485 quality management system certification, GMP certificate. SARS-CoV-2 Antigen Rapid Test Kit has been awarded CE certification, German BfArM approved self-test and professional test, PEI certification, EU general and mutual recognition list, and multinational white list. Ranked No. 1 in German authoritative medical magazine Daz and No. 1 in Hong Kong sales.

Enterprise mission: Caring for health and benefiting the society. Focus on products and lead the industry.

Core values: Inheriting innovation, improving quality, honesty and law-abiding, and win-win cooperation.

Our vision is to become a leading company in the biodiagnostic reagent industry, providing high-quality, convenient and reliable services to the world.