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Ochratoxin A (OTA) Rapid Mycotoxin Testing Kits For Grain Feed 50
Tests/Kit
1. Brief
This product is used for testing Ochratoxin residue in grain and
feed. The whole test need about 5 -10min, suitable for on-site
rapid qualitative detection. The sensitivity is5ng/ml ( 5ppb ).
2. Components
Ochratoxin A rapid test card | 50 pieces/kit |
Extract buffer (Ethyl acetate) | 1 tube |
Sample buffer | 1 tube |
15 ml centrifuge tube | 2 piece |
5 ml centrifuge tube | 4 pieces |
Manual | 1 piece |
3. Materials required but not provided
Centrifuge, micropipette, nitrogen-drying device
4. Sample preparation of grain and feed
1) Take above 5g of representative samples of feed and grain (rice,
wheat, corn, peanuts) to crush (through a 20-mesh sieve), weigh
accurately 2g crushed sample into the 15ml centrifuge tube.
2) Add accurately 3ml Extract buffer (Ethyl acetate) into the 15ml
centrifuge tube, cup and seal, shake strongly for 3min, centrifuge
at 4000 rpm for 3min.
3) Use dropper to absorb 1ml up-layer clear liquid from the 15ml
centrifuge tube into the 5ml centrifuge tube, blow to dry by
nitrogen-drying device or hair dryer (it is normal to have some
yellow fat in the bottom of tube), add 0.3ml sample buffer (about
10 drops by dropper) into the dry 5ml centrifuge tube to
re-dissolve the dry residue, the solution is the sample ready to
test.
5. Operation procedures
1) Read the instruction before use, return the test card, prepared
sample into room temperature(20℃-30℃)before testing, do not open
the foil bag without preparation.
2) Open the foil bag, take outtest card/microwell/dropper(total 3
items), put it flatly.
3) Use dropper absorb 150ul (about 5 drops) above prepared solution
into microwell, use dropper blow and beat gently for 30s, dissolve
the red materials in the bottom of microwell completely, be static,
wait to react for 2min, absorb the red solution, gently blow and
beat for 10s, then transfer all solution into “S” well on test card
and begin timing.
4) Read the result in 5-8 minutes, It is invalid beyond 30mins.
6. Test Result Interpretation
6.1 Negative (—): Both C line andT line appear, the color of T line
is darker than or same as C line, it means the Ochratoxin A
concentration is lower than 5ug/kg or no Ochratoxin A residue.
6.2 Positive (+): The color of T line is lighter than C line or T
line is invisible, it means the Ochratoxin A concentration is
higher than 5ug/kg. The lighter of T line compare with C line, the
higher concentration of Ochratoxin A in sample.
6.3 Invalid: C line is invisible, maybe it is the operation error
or test card is invalid. Read the manual, use new test card to test
again. If the problem keep happening, please stop using this lot
no. product and contact local supplier.
7. Precautions
1) Use test card for once during expiry date;
2) Avoid direct sunlight and direct blowing of fan during testing;
3) Do not touch the white membrane surface in the middle of test
card;
4) Tap water, distilled water or deionized water cannot be used as
negative control;
5) Take care to ensure that the sample is fresh and avoid failure
or contamination due to deterioration. When a positive result
occurs, it is recommended to use this card to test again or
according to legal procedures to separate the packaged samples for
confirmation testing.
6) Safety Tip: Wear gloves during the whole experiment. The used
glass and plastic bottles should wash with tap water inside and
outside. The glass bottles and plastic bottles are placed
separately in the designated special recycling bags. After the
garbage collection bags are full, notify the outside eco-car, and
recycle the waste according to the garbage classification.
8.Storage and expiry date
Storage: Store at 2-30 ℃ in dark,no frozen.
Expiry date: 18 months; date of production is on box.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by
air and ground.Please confirm with us before placing an order.