Laboratory Or Hospital High Accuracy CMV IgG Elisa Test Kit
Brand Name:BIOVANTION
Certification:ISO 13485
Model Number:TY0031
Minimum Order Quantity:1000 Box
Delivery Time:2-7 days
Payment Terms:L/C, D/A, D/P, T/T, Western Union, MoneyGram
Contact Now
Add to Cart
Verified Supplier
Location:
Beijing Beijing China
Address:
Room230/232, Building 1, No.538 YongfengTun, Haidian District, Beijing
Supplier`s last login times:
within 1 hours
Product Details
Company Profile
Product Details
CMV IgG Elisa Test Kit
INTENDED USE
CMV IgG Elisa test is intended for the detection and quantitative determination of IgG antibody to CMV in human sera.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
ASSAY PROCEDURE
- Prepare Reagents: Dilute 1 volume of Washing Buffer (20×)with 19 volumes of distilled water, mix well.
- Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 100μL of Sample Diluent , dispense 10μL of sample, 100μL negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating the plate.
- Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.
- Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
- Add Conjugate: Add 100μL of conjugate to each well (except the blank well).
- Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
- Wash the Plate: Repeat the wash procedure as in step 4.
- Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 15 minutes.
- Stop reaction: Add 50μL Stop Solution to each well, mix well.
- Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
Storage
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and
return to 2-8 °C, under which conditions the wells will remain
stable for 2 months, or until the labeled expiry date whichever is
earlier.
• Seal and return unused calibrators to 2-8 °C, under which
conditions the stability will be retained for 1 month, for longer
use, store opened calibrators in aliquots and freeze at-20 °C.
Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under
which conditions the stability will be retained for 2 months, or
until the labeled expiry date, whichever is earlier.
Laboratory Or Hospital High Accuracy CMV IgG Elisa Test Kit
Inquiry Cart
0