This ELISA kit uses Sandwich-ELISA as the method. The Microelisa
stripplate provided in this kit has been pre-coated with an
antibody specific to IL-2 . Standards or samples are added to the appropriate Microelisa
stripplate wells and combined to the specific antibody. Then a
Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-2 is added to each Microelisa stripplate well and incubated. Free
components are washed away. The TMB substrate solution is added to
each well. Only those wells that contain IL-2 and HRP conjugated thrombomodulin antibody will appear blue in
color and then turn yellow after the addition of the stop solution.
The optical density (OD) is measured spectrophotometrically at a
wavelength of 450 nm. The OD value is proportional to the
concentration of IL-2 . You can calculate the concentration of IL-2 in the samples by comparing the OD of the samples to the standard
curve.
Notes:
Store the kit at 4°C upon receipt.The kit should be equilibrated to
room temperature before the assay. Remove any unneeded strips from
IL-2 Antibody-Coated plate, reseal them in zip-lock foil and keep
at 4°C.
Precipitates may appear in concentrated washing buffer. Please heat
the buffer to dissolve all the precipitates, which will not affect
the results.
Accurate pipette should be used to avoid experimental error.
Samples should be added to the Microplate in less than 5 minutes.
If a large number of samples are included, multiple channel pipette
is recommended.
Standard curve should be included in every assay. Replicate wells
are recommended. If the OD value of the sample is greater than the
first well of standards, please dilute the sample (n times) before
test. When calculating the original thrombomodulin concentration,
please multiply the total dilution factor (XnX5).
In order to avoid cross-contamination, Microplate sealers are for
one-time use only.
Please keep Substrate away from light.
All the operation should be accordance with the manufacturer's
instructions strictly. The results determined by the Microtiter
Plate Reader.
All the samples, washing buffer and wastes should be treated as
infectious agents.
Reagents from different lots should not be mixed.
Company Profile
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world