Sheep Interleukin 1 Beta Elisa Kit IL1F2 ELISA RUO Test Kit Il1b Elisa Kit

Brand Name:BIOVANTION
Model Number:In-Sh0046
Minimum Order Quantity:1 Box
Delivery Time:5-8 working days
Payment Terms:L/C, Western Union, T/T
Place of Origin:CHINA
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Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B;IL1F2 ELISA Kit

Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B ELISA Kit


  • For research use only.
  • For the quantitative in vitro determination of Sheep Interleukin 1 Beta(IL-1B) concentrations in Sheep serum, plasma, culture media or any biological fluid.
  • Expiration date:six months .
  • Storage: 2-8℃.
  • Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1B Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-1B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1B and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1B. You can calculate the concentration of IL-1B in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit48 determinations96 determinationsStorage
1User manual11R.T.
2Closure plate membrane22R.T.
3Sealed bags11R.T.
4Microelisa stripplate112-8℃
5Standard:270pg/ml0.5ml×1 bottle0.5ml×1 bottle2-8℃
6Standard diluent1.5ml×1 bottle1.5ml×1 bottle2-8℃
7HRP-Conjugate reagent3ml×1 bottle6ml×1 bottle2-8℃
8Sample diluent3ml×1 bottle6ml×1 bottle2-8℃
9Chromogen Solution A3ml×1 bottle6ml×1 bottle2-8℃
10Chromogen Solution B3ml×1 bottle6ml×1 bottle2-8℃
11Stop Solution3ml×1 bottle6ml×1 bottle2-8℃
12Wash solution20ml (20X)×1bottle20ml (30X)×1bottle2-8℃

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1B were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1B were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%


Assay range

3pg/ml -200pg/ml


Sensitivity:

0.5pg/ml

China Sheep Interleukin 1 Beta Elisa Kit IL1F2 ELISA RUO Test Kit Il1b Elisa Kit supplier

Sheep Interleukin 1 Beta Elisa Kit IL1F2 ELISA RUO Test Kit Il1b Elisa Kit

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