Human cytokeratin 18,CK-18/Keratin, type I cytoskeletal 18 ELISA
Kit
Expiration date:6 months .
Storage: 2-8℃.
Package Size :96 tests/Kit
For the quantitative in vitro determination ofcytokeratin 18 (CK-18)concentrations inHumanserum,plasma,culturemediaor any biological fluid.
For research use only.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa
stripplate provided in this kit has been pre-coated with an
antibody specific to cytokeratin 18 (CK-18). Standards or samples
are added to the appropriate Microelisa stripplate wells and
combined to the specific antibody. Then a Horseradish Peroxidase
(HRP)- conjugated antibody specific for cytokeratin 18 (CK-18) is
added to each Microelisa stripplate well and incubated. Free
components are washed away. The TMB substrate solution is added to
each well. Only those wells that contain cytokeratin 18 (CK-18) and
HRP conjugated cytokeratin 18 (CK-18) antibody will appear blue in
color and then turn yellow after the addition of the stop solution.
The optical density (OD) is measured spectrophotometrically at a
wavelength of 450 nm. The OD value is proportional to the
concentration of cytokeratin 18 (CK-18). You can calculate the
concentration of cytokeratin 18 (CK-18) in the samples by comparing
the OD of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit
48 determinations
96 determinations
Storage
1
User manual
1
1
R.T.
2
Closure plate membrane
2
2
R.T.
3
Sealed bags
1
1
R.T.
4
Microelisa stripplate
1
1
2-8℃
5
Standard:3600pg/ml
0.5ml×1 bottle
0.5ml×1 bottle
2-8℃
6
Standard diluent
1.5ml×1 bottle
1.5ml×1 bottle
2-8℃
7
HRP-Conjugate reagent
3ml×1 bottle
6ml×1 bottle
2-8℃
8
Sample diluent
3ml×1 bottle
6ml×1 bottle
2-8℃
9
Chromogen Solution A
3ml×1 bottle
6ml×1 bottle
2-8℃
10
Chromogen Solution B
3ml×1 bottle
6ml×1 bottle
2-8℃
11
Stop Solution
3ml×1 bottle
6ml×1 bottle
2-8℃
12
Wash solution
20ml (20X)×1bottle
20ml (30X)×1bottle
2-8℃
Notes:
Store the kit at 4°C upon receipt.The kit should be equilibrated to
room temperature before the assay. Remove any unneeded strips from
Human cytokeratin 18 (CK-18)Antibody-Coated plate, reseal them in
zip-lock foil and keep at 4°C.
Precipitates may appear in concentrated washing buffer. Please heat
the buffer to dissolve all the precipitates, which will not affect
the results.
Accurate pipette should be used to avoid experimental error.
Samples should be added to the Microplate in less than 5 minutes.
If a large number of samples are included, multiple channel pipette
is recommended.
Standard curve should be included in every assay. Replicate wells
are recommended. If the OD value of the sample is greater than the
first well of standards, please dilute the sample (n times) before
test. When calculating the origina cytokeratin 18 (CK-18)
concentration, please multiply the total dilution factor (XnX5).
In order to avoid cross-contamination, Microplate sealers are for
one-time use only.
Please keep Substrate away from light.
All the operation should be accordance with the manufacturer's
instructions strictly. The results determined by the Microtiter
Plate Reader.
All the samples, washing buffer and wastes should be treated as
infectious agents.
Reagents from different lots should not be mixed.
Company Profile
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world