Human High Quality Anti-HIV 1&2 ELISA Kit 96Test/Kit The third generation double antigen sandwich method to detect
antibody to HIV1+2 in serum or plasma 1.SUMMARY AND PRINCIPLE OF THE TEST
Human Immunodeficiency Virus Type (HIV-1) has been isolated from
patients with AIDS and AIDS-related complex (ARC). HIV-1 was
thought to be the sole causive agent of these syndromes until 1986,
when a second type of Human Immunodeficiency Virus (HIV-2) was
isolated and also reported to cause AIDS. Since the initial
discovery, more than 600 cases of HIV-2 infection have been
documented worldwide, with over 40 cases of AIDS related to HIV-2.
Both viruses have the same morphology and lymphotropism, and the
modes of transmission appear to be identical. In addition, HIV-1
and HIV-2 genomes exhibit about 60% homology in conserved genes
such as gag and pol. Serologic studies have also shown that the
core proteins of HIV-1 and HIV-2 display frequent cross-reactivity
whereas the envelope proteins are more type-specific.
2. Materials provided with the kits:
Item
Description
96T
480T
1
Microtiter Well
1
5
2
Sample Diluent
6ml
30ml
3
Enzyme Conjugate
12ml
60ml
4
Negative Control
1ml
5ml
5
Positive Control(HIV-1)
1ml
5ml
6
Positive Control(HIV-2)
1ml
5ml
7
Wash Buffer Concentrate (20x)
30ml
150ml
8
Substrate Solution A
6ml
30ml
9
Substrate Solution B
6ml
30ml
10
Stop Solution
6ml
30ml
11
User manual
1 copy
5 copy
3. ASSAY PROCEDURE
Allow all components to reach room temperature before use. Dilute
concentrated wash buffer 1:20 with ddH2O.
Set two positive and three negative controls. Dispense 100μl of
Positive Control(HIV-1 and HIV-2) as well as Negative Control in
duplicate into respective wells without sample diluent. Set one
blank well as background control without adding any liquid. Add
50μl of sample diluent into each test well, add 50 μl test serum or
plasma into test wells mix thoroughly.
Place the microtiter plate into a humidified box and incubate at
37°C for 30 min.
Discard reaction solution and add 350μl diluted wash buffer to each
well. Discard the wash buffer after soaking for 15-30seconds.
Repeat the washing procedure 5 times and then inverting the plate
vigorously to get all water out and blocking the rim of wells on
absorbent paper for a few seconds.
Add 100μl of Enzyme Conjugate to each well. Mix it gently by
swirling the microtiter plate on flat bench for 1 min. Do not add
Enzyme Conjugate to the blank well..
Place the microtiter plate into a humidified box and incubate at
37°C for 30 min.
Wash each well 5 times by filling each well with diluted 1X wash
buffer, then inverting the plate vigorously to get all water out
and blocking the rim of wells on absorbent paper for a few seconds.
Add 50μl of Substrate Solution A (HRP substrate) to each well, then
add 50μl of Substrate Solution B (TMB) to each well. Mix gently and
incubate at 37°C for15 min.
Add one drop (50μl) of Stop Solution to each well to stop the color
reaction.
Read the OD value at 450 nm/630 nm with dual filter plate reader.
It is option to read the OD value at 450 nm with single filter
plate reader. (using the OD value of the blank well to correct all
the OD reading from all wells
Company Profile
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world