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Estradiol-E2
REF: DS177715 96 tests
Intended use
Immunoassay for the in vitro quantitative determination of Estradiol concentration in human serum.
Summary
Oestradiol, E2, is an oestrogen, a steroidal hormone secreted by
the ovaries. It is a main female hormone. Oestradiol regulates
female characteristics, the
maturation of female sex organs and the oestrus and menstrual
cycle. It stimulates the production of mammary glands.
The serum concentrations of oestradiol and other female hormones
change during the menstrual cycle. During gestation there is a high
serum concentrations of oestradiol, E3. During menopause, oestrone
has the higher concentration.
Oestradiol and oestrone can interchange. Oestradiol has a big
effect on bone formation. Women past menopause have an accelerated
loss of bone because they have less oestrogen.
Oestradiol also has some functions in males. Its serum
concentration in men is equivalent to that seen in post-menopausal
women.
Reagents
Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations:
0(A), 20(B), 100(C), 500(D), 1500(E) and 3000(F) pg/mL.
• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase)
labeled sheep monoclonal Progesterone in Tris-NaCl buffer
containing BSA (bovine serum albumin). Contains 0.2% ProClin300
preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine)
TMB.
• Stop Solution, 1 vial, 6.0 ml.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated),
PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent
capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with
a precision of better than 1.5%.
• Absorbent paper.
• Distilled water