Anti Tumor Necrosis Factor Rabbit Polyclonal Antibodies with 16 kDa Observed Band

Highly sensitive Rabbit Anti Tumor Necrosis Factor Polyclonal Antibody

Cat.No. BT-AP06654 Size: 100ul/50ul/20ul Datasheet Request

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,IF,ELISA

Source: Rabbit

Immunogen: Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Y9

Protein Name: 3-phosphoinositide-dependent protein kinase 1

Species reactivity: human, mouse, rat

Observed band: 16 kDa

Concentration: 1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification:

The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.

Synonyms:

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, TNFα, Tumor necrosis factor

Recommended Dilution:

IF: 1: 200 - 1: 1000

IHC: 1: 100 - 1: 300

​ELISA: 1: 10000

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.​

​Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

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Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researchers all over the world. Our products line covers a set of research areas including Immunology, Neuroscience, Cancer, Kinases, Phosphatases and Cell Biology.

Bioassay Technology Laboratory (BT Lab) as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.

Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:

ELISA and immunohistochemistry

• Immunoprecipitation
• Immunohistochemistry
• Immunofluorescence microscopy
• Immunohistochemistry
• Quantitative Multiplexing

About BT Lab

As a leading brand of Korainbio, Bioassay Technology Laboratory (BT Lab) offers a variety of cost-effective ELISA kits and sets to measure cytokines, chemokines, and soluble biomarkers consistently and reliably. Sandwich kit and Competitive that provide the core reagents our selection of ELISA products meets the demand of ELISA beginners to experts alike at a very economical price.

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ELISA Kit Categories

Sandwich Kit
Sandwich kits are fully validated and ready-to-use, containing 96- well strip plates pre-coated with capture antibody to detect sample antigen.

Competitive Kit
In a competitive ELISA assay, sample antigen and labeled antigen compete for capture antibody binding. The more target protein there is in the sample, the less labeled antigen will be captured and the weaker the signal.

Qualitative Kit
Qualitative results provide a simple positive or negative result for a sample. In quantitative ELISA, the optical density (OD) of the sample is compared to a standard curve, which is typically a serial dilution of a known-concentration solution of the target molecule. It is suitable for detecting small antigens.

Advantages of our Sandwich kit

Without Diluting Sample

• Save assay time
• Wide detection range
• Reducing non-specific interactions between the sample matrix proteins

Streptavidin-biotin system

• Significant non-specific binding can be prevented by strepvidin
• Reducing interference from residual biotin in sample
• A non-covalent interaction with high affinity

Washing Step

• Only 5 times for one step

Pre-Coated plate

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