96 Wells HIV Antibody Test Kit Biotin Working Liquid Quantitative Measurement

HIV 1/2 antibody elisa test kitt,96wells/kit, high accuracy Elisa Sandwich method, for quantitative measurement

Materials provided with the kit:

Note: different batches of reagent kit, and different component can not be exchanged for use. Once open, stable for 3 months at 2-8°C.

INTENDED USE

The Anti-HIV 1+2 ELISA is a qualitative enzyme immunoassay for the in vitro detection of Human

Immunodeficiency Virus (HIV) infection, based on the detection of antibodies to HIV-1 and HIV-2 in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with HIV.

ASSAY PROCEDURE

1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer with 19 volumes of distilled water,

mix well.

2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50ul of Sample Diluent , dispense 50 ul of sample or negative control or positive control to the respective wells. Gently vibrating the plate.

3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37 for 60 minutes.

4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted

washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.

5. Add Conjugate: Add 100ul of conjugate to each well (except the blank well).

6. Incubate: Cover the Microplate and incubate the plate at 37 for 30 minutes.

7. Wash the Plate: Repeat the wash procedure as in step 4.

8. Add Substrate: Add 50ul of Substrate Solution A and 50 ul of Substrate Solution B to each well, mix well.

9. Stop reaction: Add 50ul Stop Solution to each well, mix well.

10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should

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