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P2101 QPCR Master Mix Power Green QPCR Kit 400 Rxn 20μL Reaction

P2101 QPCR Master Mix Power Green QPCR Kit 400 Rxn 20μL Reaction

Brand Name:GDSBio
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Model Number:P2101
Minimum Order Quantity:1Bag
Delivery Time:8 work days
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Product Details

Power Green qPCR Mix

For research use only

Cat. No: P2101, 400 rxn/20μl reaction

P2102, 2000 rxn/20μl reaction

Components

ComponentP2101P2102
2X Power Green qPCR Mix1 ml1 ml × 5
Nuclease-free Water1 ml1 ml × 5

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.


Description

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively.


Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation


Features

• Compatible with many Real-time systems which not require ROX reference dye

• Exceptional specificity with dual hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility


Table of Instrument Guide

InstrumentsFinal Conc. of ROX
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700500 nM,high ROX
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx400050 nM,low ROX
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart CyclerNo ROX

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

China P2101 QPCR Master Mix Power Green QPCR Kit 400 Rxn 20μL Reaction supplier

P2101 QPCR Master Mix Power Green QPCR Kit 400 Rxn 20μL Reaction

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