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Rat pyruvate dehydrogenase (PDH) ELISA Kit

Rat pyruvate dehydrogenase (PDH) ELISA Kit

Minimum Order Quantity:1
Delivery Time:3days
Payment Terms:TT/LC
Place of Origin:china
specification:48T/96t
Price:200usd/kit
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Location: Hangzhou Zhejiang
Business type: Manufacturer, Distributor/Wholesaler
Address: Room 563 ,newtown building ,jianggan district ,Hangzhou ,China
Supplier`s last login times: within 32 hours
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Product Details

Generic Name:Rat pyruvate dehydrogenase (PDH) ELISA Kit.

Purpose

This kit allows for the determination of PDH concentrations in Rat serum,Tissue, cell culture supernatant and other biological fluids.

Principle of the assay

The kit assay Rat PDH level in the sample,use Purified Rat PDH to coat microtiter plate wells, make solid-phase antibody, then add PDH to wells, Combined PDH antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard:180 U/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

(20ml×20 fold)

×1bottle

(20ml×30 fold)

×1bottle

2-8℃

China Rat pyruvate dehydrogenase (PDH) ELISA Kit supplier

Rat pyruvate dehydrogenase (PDH) ELISA Kit

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