Convenient Cell Dissociation Solution, Recombinant Trypsin Solution
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin
solution optimized for cell dissociation. It is formulated with
recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of
recombinant human trypsin is same as human trypsin 2 sequence.
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal end of lysine and arginine amino acid
residues. YaxinBio recombinant trypsin is a genetically engineered
protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to
porcine pancreas-derived trypsin,with equivalent properties
compared to native trypsin .Recombinant trypsin can replace native
trypsin for using in a variety of biotechnological processes. The
M.W. of recombinant trypsin is 24KD,and the optimum pH is pH
7.0-11.0. The enzyme is inhibited by serine protease inhibitors,
e.g. PMSFsoybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was
found and bioburden is conform to biological standard, eliminates
the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to
native trypsin. Soybean trypsin inhibitors and other inhibitors
work the same way with recombinant human trypsin as they do with
Recombinant trypsin provides high specific activity and eliminates
contaminated material found in 1:250 trypsin or other animal origin
The trypsin solution, ready-to-use, eliminates the risk of activity
loss in the process of transport and storage. It also eliminates
the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal
concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result
can be obtained, and then eliminate EDTA influence on cell
apoptosis experimental analysis.
|Recombinant Trypsin Solution||RTS04||2000 BAEE u/ml||100 ml,500ml||YaxinBio|
UNIT DEFINITION:One BAEE unit of trypsin activity will produce a Delta A253 of
0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃
(1cm light path). 1 USP unit = 3 BAEE unit.