Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody
Column Purified Protein A
Recombinant Protein A
Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.
The recombinant Protein A is a genetically engineering protein
containing IgG-binding domains. Recombinant Protein A is ideal for
purification of polyclonal or monoclonal IgG antibodies. Protein A
binds to most human and mouse IgG subclasses (e.g., human IgG1,
IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow,
guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium
(such as agarose) for monoclonal and polyclonal antibody
purification. Recombinant protein A can be coupled to a variety of
molecules (such as fluorescent molecules, enzyme markers, biotin,
colloidal gold and radioactive markers). These coupled derivatives
can be used in antibody test in the process of Western-blot, ELISA
or immunohistochemical tests.
The C-terminus of the recombinant protein A contains cysteine which
can be coupled to the solid separation medium for purification of
the monoclonal antibody or polyclonal antibody. Recombinant protein
A can be conjugated to a variety of reporter molecules (fluorescent
dyes, enzyme labels, biotin, colloidal gold, radioactive labels,
etc.) without affecting its binding to the antibody. These
conjugated protein A derivatives can be used for antibody detection
of Western-blot, ELISA, immunohistochemistry.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be
stored under 2-8℃ in sealed container.
It is stable within 24 months.When gluing even with PBS (pH7.4)
dissolved,it is now with the current use.
After dissolved with pure water, it should be stored under -20℃.
Stability of transport: The product is stable by blue ice
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin