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Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A

Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RSPA05
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China, Shanghai, Xuhui
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A


Recombinant Protein A

CAS:91932-65-9

Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.


Application(1)


The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.


APPLICATION(2)


The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.


MAIN FEATURES

AppearanceWhite or off white powder
SolubilityClear liquid
Bioburden≤ 1 cfu/ mg
SDS-PAGESingle major band
SDS-PAGE (MW)25000±1000 Daltons
UV SpectrumE275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC)≥ 95%

STABILITY OF STORAGE AND TRANSPORT


Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

After dissolved with pure water, it should be stored under -20℃.


Stability of transport: The product is stable by blue ice insulation transport.


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Product Tags:

SPA protein A

      

purified protein

      

Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A Images

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Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified