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Trypsin Solution 1 / 2500 Animal Composition Free Trypsin for Cell Dissociation

Trypsin Solution 1 / 2500 Animal Composition Free Trypsin for Cell Dissociation

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RTS04
Minimum Order Quantity: 25ml
Delivery Time: Six days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation


Recombinant trypsin solution(RTS)

Description


Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.


Method Of Use


Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.

Step 2. Dissolve the trypsin powder.

Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.


Notes


Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.

Note 2. If necessary, after dissolving, immediately dispense into suitable ; containers, then stored under -20℃. To use it, dissolve it at room temperature.

Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.


Instructions for use


The following instructions are applicable for most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines (see notes below).

1. Wash the cells with DPBS w/o Ca, Mg (Cat No. 02-023-1).

2. Add 1ml of Recombinant Trypsin Solution to each T-25 tissue culture flask.

3. Incubate the flask at 37°C for 2 min or longer as necessary. Verify cell’s detachment using inverted microscope.

4. Pellet the cells by centrifugation, and discard the supernatant. 6. Resuspend the cells in growth medium, and seed as desired.


Product Tags:

trypsin for cell culture

      

porcine trypsin

      

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Trypsin Solution 1 / 2500 Animal Composition Free Trypsin for Cell Dissociation