Human Trypsin Solution Pre-formulated Concentration to Dissociate
Special, animal components-free (ACF) recombinant trypsin
solutions, developed as an alternative to porcine trypsin. The
solutions do not contain any chymotrypsin, carboxypeptidase A, and
other protease contaminant. Recombinant Trypsin Solution
formulations were developed for efficient dissociation of adherent
cell types from surfaces and tissues and were optimized for
sensitive cells, such as primary human mesenchymal stem cells
(hMSC). The addition of EDTA usually accelerates the dissociation
phase. Recombinant Trypsin Solutions are pure enzyme solutions,
which help maximize the yield of functionally viable cells from
culture vessels, while preventing the toxicity effect induced by
other non desirable proteases. In addition, recombinant trypsin
eliminates the risk of viruses, or other potential adventitious
agents found in animal derived components.
Animal origin free:The use of recombinant Human Trypsin eliminates
the risk of virus presence, and
of any other potential adventitious agents found in animal
pancreas-derived trypsin. YaxinBio Recombinant Human Trypsin
belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as
the Human Trypsin 2.
Stable:A sterile recombinant human trypsin lyophilized eliminates
the contamination risks and decreases the chance of activity loss
in the process of transport and storage.
1) Recombinant human trypsin provides increased specificity and
contaminating activities found in lower purity enzymes.
2) No other contaminating proteases such as chymotrypsin or
3) Purity is more than 95% by HPLC.
White or White-like lyophilized
≥ 95% by HPLC
No chymotrypsin, carboxypeptidase A, and other protease
Unit Definition:One USP unit of trypsin activity will produce a
Delta A253 of 0.003 per minute in a
reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate
(1cm light path).
Recombinant human trypsin lyophilized should be stored under 2℃-8℃
in sealed container. It is
stable within 24 months.After dissolved, it should be stored under
-20℃. It is stable within 24 months
and above 90% activity remained after 10 times repeated freezing