Cell Dissociation Solution, Recombinant Human Trypsin for Enzymatic
Hydrolysis of Protein
Cell Dissociation Solution - Recombinant Human Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal end of lysine and arginine amino acid
residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by
serine protease inhibitors, e.g. PMSF, and by metal chelating
agents, e.g., EDTA.
Recombinant Human Trypsin is a genetically engineered protein
expressed in E.coli and purified by high pressure liquid chromatography. There are no
contaminating enzyme activities such as carboxypeptidase A and
chymotrypsin. No protease inhibitors such as PMSF are contained in
Animal origin free:The use of recombinant Human Trypsin eliminates
the risk of virus presence, and of any other potential adventitious
agents found in animal pancreas-derived trypsin. YaxinBio
Recombinant Human Trypsin belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as
the Human Trypsin 2.
Stable:A sterile recombinant human trypsin lyophilized eliminates
the contamination risks and decreases the chance of activity loss
in the process of transport and storage.
1) Recombinant human trypsin provides increased specificity and
contaminating activities found in lower purity enzymes.
2) No other contaminating proteases such as chymotrypsin or
3) Purity is more than 95% by HPLC.
White or White-like lyophilized
≥ 2500 USP u/mg pro.
≥ 95% by HPLC
No chymotrypsin, carboxypeptidase A, and other protease
Unit Definition :One USP unit of trypsin activity will produce a
Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at
pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recombinant human trypsin lyophilized should be stored under 2℃-8℃
in sealed container. It is stable within 24 months.After dissolved,
it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and