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Cell Dissociation Solution, Expressed in E.coli, Dissociate Cells

Cell Dissociation Solution, Expressed in E.coli, Dissociate Cells

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China, Shanghai, Xuhui
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Cell Dissociation Solution, Expressed in E.coli, Dissociate Cells

Recombinant Trypsin Solution


Description

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.


Advantages


Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.


Main Features


Source

E. Coli

Purified by

HPLC

Specific activity

≥3800 USP units/mg pro

Purity(RP-HPLC)

NLT 70% β-trysin, NMT 20% α-trypsin

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.


Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.



Product Tags:

dissociated cell culture

      

trypsin cell culture

      

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Cell Dissociation Solution, Expressed in E.coli, Dissociate Cells