登录 | 免费注册 | 提交网站 | 免费看询盘 | 外贸旺站免费建站 | "贸易通"多语言推广 | 外贸论坛
Hot Products:
Home > Polymer >

Stable Recombinant Carboxypeptidase B, Expressed in E.coli

Stable Recombinant Carboxypeptidase B, Expressed in E.coli

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Minimum Order Quantity: 10mg
Delivery Time: 7 work days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Stable Recombinant Carboxypeptidase B, Expressed in E.coli


Recombinant Carboxypeptidase B


peptidyl-L-lysine [L-arginine]hydrolase
EC :3.4.17.2
CAS:9025-24-5
Synonyms:carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B;
Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 33.8 kDa. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Advantages


Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B


Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.


High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.


Main Features


Format

White or white-like or yellowish lyophilized

Additives

Tris salts, NaCl salts and carbohydrates

Protein content

35% ~ 60%

Specific activity

≥170 units/mg pro.

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.


Unit Definition


One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.


RELATED PRODUCTS

Recombinant enterokinase

Recombinant V8 protease

Sequencing grade chymotrypsin

Sequencing grade carboxypeptidase B



Product Tags:

sequencing grade modified trypsin

      

carboxypeptidase enzyme

      

Stable Recombinant Carboxypeptidase B, Expressed in E.coli Images

Send your message to this supplier
From:
Enter your email please.
To: Shanghai Yaxin Biotechnology Co.,Ltd.
Subject:
Message:
Characters Remaining: (0/3000)
 
Inquiry Cart 0
Other about

Stable Recombinant Carboxypeptidase B, Expressed in E.coli