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Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCT03
Minimum Order Quantity: 100ug
Delivery Time: 7 days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution


Descirption


Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

CAS: 9004-07-3
EC: 3.4.21.1

Source: human chymotrypsin, expressed in E. Coli.


Main Features


Source

E. Coli

M.W.

26,950 Da

Purity

≥ 98 % by HPLC.

Format

Lyophilized

Specific activity

≥ 3000 units/mg pro


Application


1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.


Recommend Usage


For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.


Storage Instructions


Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.


Product Tags:

sequencing grade modified trypsin

      

serine protease

      

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution Images

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Sequencing Grade Chymotrypsin for Digestion Reactions In-solution