Animal Origin Free, Carboxypeptidase B, For Protein Structure and
Recombinant Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, the pH optimum is
8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited
by arginine and lysine. The enzyme is also inhibited by metal
chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is
expressed in E.Coli and purified by high pressure liquid
chromatography. There is no trace of other enzyme (such as
carboxypeptidase A and chymotrypsin) activity. No protease
inhibitors such as PMSF are present in the preparation.
Purity by HPLC
≥ 200 units/mg pro
No chymotrypsin, carboxypeptidase A, or other proteases
Protein structure and sequence analysis, such as hydrolyze basic
amino acids lysine, arginine and histidine from the C-terminal end
Antibody quality control.
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and no activity lose after 10 times
repeated freezing and thawing.