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Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis

Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China, Shanghai, Xuhui
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis


Recombinant Carboxypeptidase B

Description


Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.


Main Features


Purity by HPLC

≥ 95%

Format

Lyophilized

Specific activity

≥ 200 units/mg pro

Contaminant activity

No chymotrypsin, carboxypeptidase A, or other proteases contaminant.


Application


Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.


Recommend Usage


Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.


Product Tags:

trypsin endopeptidase

      

carboxypeptidase enzyme

      

Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis Images

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Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis