96 Wells 48 Wells Mouse Strong Specificity Cathelicidin Antimicrobial Peptide ELISA Kit
Brand Name:BT Lab
Certification:CE, ISO9001:2005, MSDS
Model Number:Cat.No E1739Mo
Minimum Order Quantity:Negotiation
Delivery Time:1-3 business days, bulk order within one week
Payment Terms:Western Union, T/T
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Product Details
96 Wells 48 Wells Mouse Strong Specificity Cathelicidin
Antimicrobial Peptide ELISA Kit
Cat.No E1739Mo
Standard Curve Range: 0.05ng/ml - 15ng/ml
Sensitivity: 0.02ng/ml
Size: 96 wells
Precision
Intra-Assay Precision (Precision within an assay) Three samples of
known concentration were tested on one plate to assess intra-assay
precision.
Inter-Assay Precision (Precision between assays) Three samples of
known concentration were tested in separate assays to assess
inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Reagent Provided
| Components | Quantity |
| Standard Solution (16ng/ml) | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (30x) | 20ml x1 |
| Biotin-Conjugate Anti-Mouse cAMP/LL-37 Antibody | 1ml x1 |
| User Instruction | 1 |
| Plate Sealer | 2 pics |
| Zipper bag | 1 |
Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature.
Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant.
Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C
within 30 minutes of collection.
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for
approximately 20 minutes. When collecting pleuroperitoneal fluid
and cerebrospinal fluid, please follow the procedures
above-mentioned.
Cell Culture Supernatant Collect by sterile tubes when examining secrete components.
Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect
the supernatants carefully. When examining the components within
the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the
cell concentration of approximately 1 million/ml. Damage cells
through repeated freeze-thaw cycles to let out the inside
components. Centrifuge at 2000-3000 RPM for approximately 20
minutes.
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly
and weigh before homogenization. Mince tissues and homogenize them
in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or
freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20
minutes.
*Sample can't be diluted with this kit. Owing to the the material we
use to prepare the kit, the sample matrix interference may falsely
depress the specificity and accuracy of the assay.
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (16ng/ml) with 120μl of
standard diluent to generate a 8ng/ml standard stock solution.
Allow the standard to sit for 15 mins with gentle agitation prior
to making dilutions. Prepare duplicate standard points by serially
diluting the standard stock solution (8ng/ml) 1:2 with standard
diluent to produce 4ng/ml, 2ng/ml, 1ng/ml and 0.5ng/ml solutions.
Standard diluent serves as the zero standard(0 ng/ml). Any
remaining solution should be frozen at -20°C and used within one
month. Dilution of standard solutions suggested are as follows:
| 8ng/ml | Standard No.5 | 120μl Original Standard + 120μl Standard Diluent |
| 4ng/ml | Standard No.4 | 120μl Standard No.5 + 120μl Standard Diluent |
| 2ng/ml | Standard No.3 | 120μl Standard No.4 + 120μl Standard Diluent |
| 1ng/ml | Standard No.2 | 120μl Standard No.3 + 120μl Standard Diluent |
| 0.5ng/ml | Standard No.1 | 120μl Standard No.2 + 120μl Standard Diluent |
| Standard Concentration | Standard No.5 | Standard No.4 | Standard No.3 | Standard No.2 | Standard No.1 |
| 16ng/ml | 8ng/ml | 4ng/ml | 2ng/ml | 1ng/ml | 0.5ng/mll |
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or
distilled water to yield 500 ml of 1x Wash Buffer. If crystals have
formed in the concentrate, mix gently until the crystals have
completely dissolved.
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.
96 Wells 48 Wells Mouse Strong Specificity Cathelicidin Antimicrobial Peptide ELISA Kit
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