40T/Kit Sperm Function Test Kit For Determinate Protein Tyrosine Phosphorylation
Brand Name:BRED
Certification:CE, ISO13485
Model Number:BRED-024
Minimum Order Quantity:1 kits
Delivery Time:3 - 5 working days
Payment Terms:T/T
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Shenzhen Guangdong China
Address:
Room 401, Section 2, Bld 25, Keyuanxi Industrial Park, Nanshan District, Shenzhen City GD, China
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Product Details
Male Infertility Diagnosis Sperm Function Kit for determination of Protein Tyrosine Phosphorylation of Capacitated Sperm
The kit is mainly used for detecting the protein tyrosine phosphorylation lever in mammalian sperm capacitation.
Fluorescent Staining. A series of complicated biochemical and molecular response of enhance fluidity of serosa, hyper activated movement and cAMP activate after sperm capacitation. Which made sperm tail proceed protein tyrosine phosphorylation. In which the sperm can combine with antibody of Anti-Tyrosine Phosphorylation signed by fluoresceine isothiocyanate (FITC). Green fluorescence can be detected in sperm tail at excitation wavelength between 450nm – 490nm.
Features:
- This is an alternative test for Sperm - zona pellucida binding test, which can reflect the binding ability of the sperm with zona pellucida.
- Help to choose the assisted reproductive way scientifically
- Double marking technology, easy to observe results
- 40T/Kit
- CE approved
Storage Condition:
- Dedicated tissue slides, Long shaped coverslip and glass slide barrel can keep at room temperature.
- Divide the unseal Krebs-Ringer Medium of sperm into several parts and store at -20°C, freeze-thawing repeatedly is not allow.
- Other reagent need to keep in dark place (2-8°C).
Period of validity:
For professional use only 1/6
- The kit store at 2-8°C ,period of validity is 2 years.
- The reagent store at -20°C, the period of validity for using is the same as the kit`s.
- Once unsealed, the kit would be kept for 45 days at 2-8° C.
Applicable instruments:
- Fluorescence microscope
Other equipment:
- Refrigerator (2-8°C)
- Freezer (-20°C)
- Water bath (37°C)
- Low velocity centrifugal machine
- Optical microscope
- Carbon dioxide incubator
- Bechtop
- Counting device of sperm
Other materials required but not provided
- Semen sampling device
- Liquefier
- Digital adjustable pipette and disposable tips (1-10μl)
- Disposable plastic tubularis
- Glass tube (5ml)
- Cone-shape centrifuge tube (15ml)
- Eppendorf tube
- Wet box
40T/Kit Sperm Function Test Kit For Determinate Protein Tyrosine Phosphorylation
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