Cell Dissociation Solution, Specific Activity ≥3800 USP Units/mg
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin
solution optimized for cell dissociation. It is formulated with
recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of
recombinant human trypsin is same as human trypsin 2 sequence.
|Appearance||Clear, Colorless to Yellowish solution|
|Composition||10mM HCl,20mM CaCl2, pH2.0-3.0|
|Protein Concentration||70 ±10 mg/ml|
|Specific activity||≥ 3800 USP units/mg pro|
|Purity(RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003
per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with
BAEE as a substrate (1cm light path).
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free,eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was
found and bioburden is conform to biological standard, eliminates
the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to
native trypsin. Soybean trypsin inhibitors and other inhibitors
work the same way with recombinant human trypsin as they do with
Recombinant trypsin provides high specific activity and eliminates
contaminated material found in 1:250 trypsin or other animal origin
The trypsin solution, ready-to-use, eliminates the risk of activity
loss in the process of transport and storage. It also eliminates
the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal
concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result
can be obtained, and then eliminate EDTA influence on cell
apoptosis experimental analysis.