Recombinant Carboxypeptidase B, Catalyzes Hydrolysis of Basic Amino
Acids Lysine, Arginine and Histidin
Recombinant Carboxypeptidase B
Recombinant Carboxypeptidase B
Storage temperature: 2-8℃
Source: Recombinant carboxypeptidase B is a genetically engineered protein
expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is
identical to rat carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the
optimum pH is 7.5-9.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA. YaxinBio has cloned rat
carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal
original carboxypeptidase B .Recombinant carboxypeptidase B can
replace native carboxypeptidase B for using in a variety of
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥170 units/mg pro|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole
ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water
or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to
1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized
should be stored under 2-8℃ in sealed container. It is stable
within 24 months.After dissolved wth sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and
Stability of transport: The product is stable by bue ice insulation transport.
(1)Animal origin free: Recombinant carboxypeptidase B is no
exogenous virus contamination,and any animal origin material is not
used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous
batch production.It is no difference between the batch and the
product quality is stable.
(3)High purity: Higher specific activity.No other contaminating
proteases such as chymotrypsin and carboxypeptidase A.Host protein
residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is
convenient to store and transport.
Compliance with regulatory requirements: Production equipment and
production environment comply with relevant regulatory
requirements, and the production process is in full compliance with
NSF ISO 9001: 2015 quality system and GMP guidelines.
(5)Complete quality documents: we can provide relevant regular
support files in according to customers’ requirement.
The production of recombinant insulin and its analogues; the
quality test of recombinant antibodies; the determination of
C-terminal amino acids of proteins; the production of other
recombinant polypeptides; and the enzymatic synthesis of certain
Sequencing grade trypsin
Recombinant human trypsin