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ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Minimum Order Quantity: 1G
Delivery Time: 3-10 DAY
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Stability of RCPB solution under -20 and repeated freezing-thawing


ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u


Recombinant Carboxypeptidase B


REAGENT


1) Reagent A. 25 mM Tris-HCl Buffer with 100 mM Sodium Chloride, pH 7.65 at 25°C±0.5°C. (Prepare 100 ml in deionized water using Tris Hydrochloride, and Sodium Chloride. Adjust to pH 7.65 at 25°C with 1 M NaOH or 1M HCl.)

2) 1.0 mM Hippuryl-L-Arginine Solution (Hippuryl-L-Arg)

Weight 16.7 mg Hippuryl-L-Arginine (Sigma Prod. No. H-2508), dissolve in 50 ml Reagent A to obtain 1.0 mM (Hippuryl-L-Arg). PREPARE FRESH.).

3) RCPB Solution

Three batches RCPB lyophilized powder (RC110919, RC110106 and RC110304), prepare 10 mg/ml with reagent A, respectively, and separated into 30 tubes for each batch, stored under -20℃.


METHOD:


Long term stability of RCPB solution: The initial activity was measure just after preparation and taken as 100%. The activity was measure every 2 months, 3 parallel samples for each batch each time, take average, calculate residual rate of activity compared with initial one and then draw a stability curve.

Stability of RCPB solution during 10 cycles repeated freezing and thawing:The initial activity was measure just after preparation and taken as 100%. The activity of 3 samples of three batches were measured according SOP just after taking from -20℃ and thawing in room temperature. Repeated 10 times. Calculate residual rate of activity compared with initial one and then draw a stability curve.


CONCLUSION:


RCPB solution (10mg/ml) is stable when kept under -20℃ in 1 year.

RCPB solution (10mg/ml) is stable during 10 cycles repeated freezing and thawing.


Fig 1. Long term stability of RCPB solution (10mg/ml) for 12 months kept under -20℃


Fig 2. Stability of RCPB solution (10mg/ml) during 10 cycles repeated freezing and thawing



Product Tags:

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trypsin endopeptidase

      

ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u Images

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