33.8 kD Molecular Weight, Recombinant Carboxypeptidase B, Expressed in E.Coli, Purified By HPLC
| Brand Name: | YAXINBIO |
|---|---|
| Certification: | NSF ISO 9001:2015 |
| Model Number: | 9025-24-5 |
| Minimum Order Quantity: | 10mg |
| Delivery Time: | 3-9 DAYS |
| Payment Terms: | T/T |
| Shanghai Yaxin Biotechnology Co.,Ltd. |
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Verified Supplier
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Shanghai Shanghai China
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| Business type: Manufacturer Exporter Seller |
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No.466 YinDu Road,XuHui, Shanghai, China
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33.8 kD Molecular Weight, Recombinant Carboxypeptidase B, Expressed in E.Coli, Purified By HPLC
Recombinant Carboxypeptidase B
Catalog Number: RCPB01
EC: 3.4.17.2
CAS:9025-24-5
Form: white to off white, yellowish lyophilized powder.
Storage condition: Recombinant Carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.
Usage:For Research or Manufacturing Purpose Only. Not for Human.
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
Function:
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8 kD, the pH optimum is 8.0, and pI is 6.0.Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
Recombinant Carboxypeptidase B(EC 3.4.17.2) is expressed in E.Coli andpurified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Protein sequence of recombinant carboxypeptidase B
Protein Sequence of Recombinant Carboxypeptidase B (rat pancreas):
UniProtKB: NP_036665.2
ASGHSYTKYNKWETIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKTRPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNIDGYVYTWTKDRMWRKTRSTMAGSSCLGVDPNRNFNAGWCEVGASRSPCSETYCGPAPESEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQIRQTCEETMLAVKYIAN YVREHLY
Product Tags:
trypsin endopeptidase
carboxypeptidase enzyme
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33.8 kD Molecular Weight, Recombinant Carboxypeptidase B, Expressed in E.Coli, Purified By HPLC Images |
