Recombinant Aprotinin, Expressed in E.coli, BPTI Inhibit
Kallikrein, CAS 9087-70-1
Aprotinin is a competitive serine protease inhibitor that inhibits
trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms
stable complexes with and blocks the active sites of enzymes.
Binding is reversible with most aprotinin-protease complexes and
dissociating at pH <3.0. Effective concentration is equimolar
with protease. YaxinBio recombinant aprotinin is a genetically
engineered protein expressed in E.coli and purified by
multi-column.The amino acid sequence of recombinant trypsin is
identical to bovine lung derived aprotinin,with equivalent
properties compared to native aprotinin and can replace native
trypsin for using in a variety of biotechnological processes.
|Source||Recombinant E. coli|
|Appearance||Clear, colorless to yellowish liquid|
|Protein Concentrate||≥ 5.0|
|Specific Activity (EPU /mg protein)||≥ 3.0|
|Absorbance (277nm)||≤ 0.8(3 EPU/ml in water)|
UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2
trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol
of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25
A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit
Recombinant Animal Components Free
Recombinant aprotinin is expressed in E. Coli, and purified by HPLC. It contains no animal-derived components.
This is a recombinant form of bovine lung aprotinin, which is
traditionally isolated from bovine lung by methods involving
fractional precipitation, gel filtration, and ion exchange
≥ 3.0 EPU/mg pro.
No any other protease contaminant.
Unit Definition:One trypsin inhibitor unit (EPU) will decrease the
activity of 2 trypsin units by 50% where one trypsin unit will
hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec
at pH 7.6 at 25 °C.A conversion factor for Aprotinin is: 1 EPU= 1
USP Aprotinin Unit = 1800 KIU.