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SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results

SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results

Brand Name: YaxinBio
Certification: NSF ISO 9001 2015
Model Number: RSPA05
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours


SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results


Recombinant Protein A

Application


The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.


Product Specification


Test/Method

Specification

Coomassie Stain

25kD

UV Spectrum

E275/250 ≥1.2,

E0.1% at 275nm=0.17-0.23

UV spectrometry

No DNA or RNA detected

Triton Content

No Triton contaminant

Format

Lyophilized

pH stable range

pH 1-13


STABILITY OF STORAGE AND TRANSPORT

  1. Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.After dissolved with pure water, it should be stored under -20℃.
  2. Stability of transport: The product is stable by blue ice insulation transport.

APPLICATION


The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.




Product Tags:

SPA protein A

      

protein A column

      

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SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification

Recombinant protein A manufacturer Antibody Test SPA Binding to IgG

Brand Name :YaxinBio
Certification :NSF ISO 9001-2015