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Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RSPA05
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification


Recombinant protein A


Application


The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.


AppearanceWhite or off white powder
SolubilityClear liquid
Bioburden≤ 1 cfu/ mg
SDS-PAGESingle major band
SDS-PAGE (MW)25000±1000 Daltons
UV SpectrumE275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC)≥ 95%

PRODUCT ADVANTAGE

  • Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
  • SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
  • The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
  • Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
  • High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
  • Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
  • Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
  • Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

Storage Instructions

Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.


Usage

For Research or Manufacturing Purpose Only. Not for Human.


Product Tags:

SPA protein A

      

purified protein

      

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification Images

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Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification