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EC No. 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

EC No. 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Minimum Order Quantity: 100U
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

EC No. 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier


Recombinant Enterokinase


Description


Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.


Common Components Influence The Action of Enterokinase


>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of Enterokinase in reaction system or extend the reaction time.


Main Features


M.W.

25,850 Da

Specific Activity

One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 16 to 24 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

Stability

Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.


Product Information


Product

Activity

Packaging

Manufacturer

Recombinant Enterokinase

≥5 u/μl

100u;500u;1ku; 10ku;20ku;100ku

YaxinBio


Unit Definition


One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.



Product Tags:

bovine enterokinase

      

enterokinase enzyme

      

EC No. 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier Images

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EC No. 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier